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Promoter replication of grape MYB transcription factor is associated with a new red flesh phenotype

文献类型： 外文期刊

作者： Li, Hui 1 ; Yang, Yaxin 1 ; Zhang, Wen 2 ; Zheng, Huan 1 ; Xu, Xianbin 1 ; Li, Haoran 1 ; Sun, Chenxu 1 ; Hu, Haipeng 1 ; Zhao, Wanli 1 ; Ma, Ruiyang 1 ; Tao, Jianmin 1 ;

作者机构： 1.Nanjing Agr Univ, Sanya Inst, Coll Hort, Nanjing 210095, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Hort Crops, Urumqi 830001, Xinjiang, Peoples R China

3.Weifang Univ, Coll Seed & Facil Agr Engn, Weifang 261061, Peoples R China

关键词： Teinturier grape; Anthocyanin; VvMYBA1; Repetitive fragment

期刊名称：PLANT CELL REPORTS （ 影响因子：6.2； 五年影响因子：5.9 ）

ISSN： 0721-7714

年卷期： 2024 年 43 卷 6 期

页码：

收录情况： SCI

摘要： In grapes, MYBA1 and MYBA2 are the main genetic factors responsible for skin coloration which are located at the color loci on chromosome 2, but the exact genes responsible for color have not been identified in the flesh. We used a new teinturier grape germplasm 'ZhongShan-HongYu' (ZS-HY) which accumulate anthocyanin both in skin and flesh as experimental materials. All tissues of 'ZS-HY' contained cyanidin 3-O-(6 ''-p-coumaroyl glucoside), and pelargonidins were detected in skin, flesh, and tendril. Through gene expression analysis at different stage of flesh, significant differences in the expression levels of VvMYBA1 were found. Gene amplification analysis showed that the VvMYBA1 promoter is composed of two alleles, VvMYBA1a and 'VvMYBA1c-like'. An insertion of a 408 bp repetitive fragment was detected in the allele 'VvMYBA1c-like'. In this process, we found the 408 bp repetitive fragment was co-segregated with red flesh and foliage phenotype. Our results revealed that the 408 bp fragment replication insertion in promoter of 'VvMYBA1c-like' was the target of its protein, and the number of repeat fragments was related to the increase of trans-activation of VvMYBA1 protein. The activation of promoter by VvMYBA1 was enhanced by the addition of VvMYC1. In addition, VvMYBA1 interacted with VvMYC1 to promote the expression of VvGT1 and VvGST4 genes in 'ZS-HY'. The discovery of this mutation event provides new insights into the regulation of VvMYBA1 on anthocyanin accumulation in red-fleshed grape, which is of great significance for molecular breeding of red-fleshed table grapes.

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