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Plastidial Phosphoglucomutase (pPGM) Overexpression Increases the Starch Content of Transgenic Sweet Potato Storage Roots

文献类型： 外文期刊

作者： Wang, Yannan 1 ; Zhang, Huan 2 ; Li, Yan 3 ; Zhang, Qian 4 ; Liu, Qingchang 2 ; Zhai, Hong 2 ; Zhao, Ning 2 ; Yang, Yufeng 5 ; He, Shaozhen 2 ;

作者机构： 1.Henan Acad Agr Sci, Cereal Crops Res Inst, Zhengzhou 450002, Peoples R China

2.China Agr Univ, Coll Agron & Biotechnol, Key Lab Sweet Potato Biol & Biotechnol, Beijing Key Lab Crop Genet Improvement,Minist Agr, Beijing 100193, Peoples R China

3.Southwest Univ, Coll Agron & Biotechnol, Chongqing 400715, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Food Crops, Prov Key Lab Agrobiol, Nanjing 210014, Peoples R China

5.Zhengzhou Univ, Henan Acad Agr Sci, Postgrad T&R Base, Cereal Crops Res Inst, Zhengzhou 450002, Peoples R China

6.Zhengzhou Univ, Sch Agr Sci, Zhengzhou 450001, Peoples R China

关键词： sweet potato; IbpPGM; chloroplast localization; overexpression; increased starch content; altered soluble sugar levels

期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）

ISSN：

年卷期： 2022 年 13 卷 12 期

页码：

收录情况： SCI

摘要： Sweet potato (Ipomoea batatas), an important root crop, has storage roots rich in starch that are edible and serve as a raw material in bioenergy production. Increasing the storage-root starch contents is a key sweet potato breeding goal. Phosphoglucomutase (PGM) is the catalytic enzyme for the interconversion of glucose-6-phosphate and glucose-1-phosphate, precursors in the plant starch synthetic pathway. Plant PGMs have plastidial and cytosolic isoforms, based on their subcellular localization. Here, IbpPGM, containing 22 exons and 21 introns, was cloned from the sweet potato line Xu 781. This gene was highly expressed in the storage roots and leaves, and its expression was induced by exogenous sucrose treatments. The mature IbpPGM protein was successfully expressed in Escherichia coli when a 73-aa chloroplastic transit peptide detected in the N-terminus was excised. The subcellular localization confirmed that IbpPGM was localized to the chloroplasts. The low-starch sweet potato cultivar Lizixiang IbpPGM-overexpression lines showed significantly increased starch, glucose, and fructose levels but a decreased sucrose level. Additionally, the expression levels of the starch synthetic pathway genes in the storage roots were up-regulated to different extents. Thus, IbpPGM significantly increased the starch content of the sweet potato storage roots, which makes it a candidate gene for the genetic engineering of the sweet potato.

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