Contribution of two-component response regulator OmpR to virulence, motility, exopolysaccharide production, and osmotic stress in Pseudomonas syringae pv. actinidiae
文献类型： 外文期刊
作者： Zhang, Lei 1 ; Chang, Xiaoxi 1 ; Zhang, Ke 1 ; Wang, Qian 1 ; Fu, Min 1 ; Huang, Lili 3 ; Zhang, Lixin 1 ;
作者机构： 1.Anhui Agr Univ, Coll Plant Protect, Anhui Prov Key Lab Integrated Pest Management Crop, Hefei 230036, Peoples R China
2.Tianjin Acad Agr Sci, State Key Lab Vegetable Biobreeding, Tianjin 300192, Peoples R China
3.Northwest A&F Univ, Coll Plant Protect, State Key Lab Crop Stress Resistance & High Effici, Yangling 712100, Peoples R China
关键词：
期刊名称：PHYTOPATHOLOGY RESEARCH （ 影响因子：3.5； 五年影响因子：3.9 ）
ISSN： 2096-5362
年卷期： 2025 年 7 卷 1 期
页码：
收录情况： SCI
摘要： Bacterial canker disease caused by Pseudomonas syringae pv. actinidiae (Psa) is the most devastating disease in kiwifruit cultivation. The EnvZ/OmpR two-component system (TCS) has been confirmed to regulate virulence and mediate environmental stress responses in Gram-negative bacteria. However, the functional role of EnvZ/OmpR in Psa has not been fully clarified. In this study, we constructed markerless ompR, envZ, and ompR-envZ mutants, and ompR complementation and overexpression strains using homologous recombination. The deletion of ompR or envZ tremendously reduced the swimming and swarming motility of Psa, as well as tolerance to osmotic stress, while overexpression of ompR impaired its virulence against kiwifruit but enhanced exopolysaccharide production. EnvZ negatively regulated hrpR/S expression in both King's B and minimal medium, whereas OmpR regulated hrpR/S expression negatively in King's B and positively in minimal medium. However, OmpR did not regulate the expression of genes gacA, algU, lpxC, fur, and fleQ, which are associated with known virulence functions, despite its binding to their promoters. Additionally, based on bioinformatic prediction, two new OmpR regulons (envC and tolQ) related to virulence were identified in Psa. Meanwhile, OmpR directly bound to the promoters of envC and tolQ, and negatively regulated their expression in minimal medium. These findings enrich our understanding of the OmpR-mediated regulatory network and its roles in the pathogenesis of P. syringae.
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