Purification, structural characteristics and anti-atherosclerosis activity of a novel green tea polysaccharide
文献类型： 外文期刊
作者： Zheng, Chao 1 ; Chen, Shun 1 ; Deng, Yuan-Yuan 3 ; Qian, Xin-Ping 1 ; Chen, Ying-Ying 1 ; Hong, Cheng-Zhi 1 ; Zeng, Ya-Fan 1 ; Li, Qiang-Ming 1 ; Pan, Li-Hua 1 ; Luo, Jian-Ping 1 ; Li, Xue-Ying 1 ; Zha, Xue-Qiang 1 ;
作者机构： 1.Hefei Univ Technol, Engn Res Ctr Bioproc, Sch Food & Biol Engn, Minist Educ, 193 Tunxi Rd, Hefei 230009, Peoples R China
2.Hefei Univ Technol, Sch Food & Biol Engn, 193 Tunxi Rd, Hefei 230009, Peoples R China
3.Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Guangzhou 510610, Peoples R China
关键词： Green tea; Polysaccharide; Chemical structure; Anti-atherosclerosis
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）
ISSN： 0141-8130
年卷期： 2024 年 254 卷
页码：
收录情况： SCI
摘要： A new homogeneous polysaccharide (TPS3A) was isolated and purified from Tianzhu Xianyue fried green tea by DEAE-52 cellulose and Sephacryl S-500 column chromatography. Structural characterization indicated that TPS3A mainly consisted of arabinose, galactose, galacturonic acid and rhamnose in a molar ratio of 5.84: 4.15: 2.06: 1, with an average molecular weight of 1.596 x 104 kDa. The structure of TPS3A was characterized as a repeating unit consisting of 1,3-Galp, 1,4-Galp, 1,3,6-Galp, 1,3-Araf, 1,5-Araf, 1,2,4-Rhap and 1-GalpA, with two branches on the C6 of 1,3,6-Galp and C2 of 1,2,4-Rhap, respectively. To investigate the preventive effects of TPS3A on atherosclerosis, TPS3A was administered orally to ApoE-deficient (ApoE-/-) mice. Results revealed that TPS3A intervention could effectively delay the atherosclerotic plaque progression, modulate dyslipidemia, and reduce the transformation of vascular smooth muscle cells (VSMCs) from contractile phenotype to synthetic phenotype by activating the expression of contractile marker alpha-smooth muscle actin (alpha-SMA) and inhibiting the expression of synthetic marker osteopontin (OPN) in high-fat diet-induced ApoE-/-mice. Our findings suggested that TPS3A markedly alleviated atherosclerosis by regulating dyslipidemia and phenotypic transition of VSMCs, and might be used as a novel functional ingredient to promote cardiovascular health.
- 相关文献
作者其他论文 更多>>
-
Tea Polysaccharide Ameliorates High-Fat Diet-Induced Renal Tubular Ectopic Lipid Deposition via Regulating the Dynamic Balance of Lipogenesis and Lipolysis
作者：Kuang, Dan-Dan;Li, Xue-Ying;Qian, Xin-Ping;Zhang, Ting;Li, Qiang-Ming;Luo, Jian-Ping;Zha, Xue-Qiang;Kuang, Dan-Dan;Li, Xue-Ying;Qian, Xin-Ping;Zhang, Ting;Li, Qiang-Ming;Luo, Jian-Ping;Zha, Xue-Qiang;Deng, Yuan-Yuan
关键词：tea; polysaccharide; renal tubular ELD; lipogenesis and lipolysis
-
Preparation and characterization of lotus root starch based bioactive edible film containing quercetin-encapsulated nanoparticle and its effect on grape preservation
作者：Zeng, Ya-Fan;Chen, Ying-Ying;Zheng, Chao;Hong, Cheng-Zhi;Li, Qiang-Ming;Yang, Xue-Fei;Pan, Li-Hua;Luo, Jian-Ping;Li, Xue-Ying;Zha, Xue-Qiang;Zeng, Ya-Fan;Chen, Ying-Ying;Zheng, Chao;Hong, Cheng-Zhi;Li, Qiang-Ming;Yang, Xue-Fei;Pan, Li-Hua;Luo, Jian-Ping;Li, Xue-Ying;Zha, Xue-Qiang;Deng, Yuan-Yuan
关键词：Bioactive edible film; Carboxymethyl lotus root starch; Quercetin; Nanoparticles; Grape preservation
-
Inhibition of Ca2+-calpain signaling is a new mechanism using Laminaria japonica polysaccharide to prevent macrophage foam cell formation and atherosclerosis
作者：Li, Xue-Ying;Kuang, Dan-Dan;Guo, An-Jun;Pan, Li-Hua;Li, Qiang-Ming;Luo, Jian-Ping;Zha, Xue-Qiang;Li, Xue-Ying;Kuang, Dan-Dan;Guo, An-Jun;Pan, Li-Hua;Li, Qiang-Ming;Luo, Jian-Ping;Zha, Xue-Qiang;Zha, Xue-Qiang;Deng, Yuan-Yuan;Deng, Yuan-Yuan;Deng, Yuan-Yuan
关键词：
-
Polygonatum cyrtonema Hua polysaccharide exhibits anti-fatigue activity via regulating osteocalcin signaling
作者：Shen, Wen-Di;Li, Xue-Ying;Zha, Xue-Qiang;Pan, Li-Hua;Li, Qiang-Ming;Luo, Jian-Ping;Shen, Wen-Di;Li, Xue-Ying;Zha, Xue-Qiang;Luo, Jian-Ping;Deng, Yuan-Yuan;Zha, Xue-Qiang;Deng, Yuan-Yuan;Deng, Yuan-Yuan
关键词：Polygonatum cyrtonema; Polysaccharide; Anti-fatigue; Osteocalcin
-
Comparison of the Effects of Different Food-Grade Emulsifiers on the Properties and Stability of a Casein-Maltodextrin-Soybean Oil Compound Emulsion
作者：Liu, Yuan;Wei, Zhen-Cheng;Deng, Yuan-Yuan;Zhang, Yan;Tang, Xiao-Jun;Li, Ping;Liu, Guang;Zhang, Ming-Wei;Liu, Yuan;Dong, Hao
关键词：food-grade emulsifiers; compound emulsion; succinylated monoglyceride; emulsion properties; emulsion stability
-
Characterization of recombinant rice quiescin sulfhydryl oxidase and its improvement effect on wheat flour-processing quality
作者：Du, Nian;Wei, Zhen-Cheng;Deng, Yuan-Yuan;Zhang, Yan;Tang, Xiao-Jun;Li, Ping;Zhang, Ming-Wei;Liu, Guang;Du, Nian;Huang, Yan-Bo;Zeng, Qiao-Hui;Wang, Jing-Jing
关键词：Quiescin sulfhydryl oxidase; Enzymatic properties; Viscoelasticity; Gluten network; Steamed bread
-
Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp
作者：Yi, Yang;Huang, Fei;Zhang, Ming-Wei;Zhang, Rui-Fen;Deng, Yuan-Yuan;Wei, Zhen-Cheng;Yi, Yang;He, Jing-Ren
关键词：longan pulp;polysaccharide;solution property;conformation;anti-tumor activity
