文献类型： 外文期刊

作者： Ding, Shanwen ¹ ; Ma, Zijun ¹ ; Yu, Lin ¹ ; Lan, Guobing ¹ ; Tang, Yafei ¹ ; Li, Zhenggang ¹ ; He, Zifu ¹ ; She, Xiaoman ¹ ;

作者机构： 1.Guangdong Acad Agr Sci, Key Lab Green Prevent & Control Fruits & Vegetable, Guangdong Prov Key Lab High Technol Plant Protect, Plant Protect Res Inst ,inist Agr & Rural Affairs, Guangzhou 510640, Guangdong, Peoples R China

关键词： Ralstonia pseudosolanacearum; Sequevar; Genome; Genetic diversity; Sunflower

期刊名称：BMC GENOMICS （ 影响因子：4.4； 五年影响因子：4.7 ）

ISSN： 1471-2164

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCI

摘要： Background Bacterial wilt caused by Ralstonia solanacearum species complex (RSSC) is one of the devastating diseases in crop production, seriously reducing the yield of crops. R. pseudosolanacearum, is known for its broad infrasubspecific diversity and comprises 36 sequevars that are currently known. Previous studies found that R. pseudosolanacearum contained four sequevars (13, 14, 17 and 54) isolated from sunflowers sown in the same field. Results Here, we provided the complete genomes and the results of genome comparison of the four sequevars strains (RS639, RS642, RS647, and RS650). Four strains showed different pathogenicities to the same cultivars and different host ranges. Their genome sizes were about 5.84 5.94 Mb, encoding 5002 5079 genes and the average G + C content of 66.85% 67%. Among the coding genes, 146 159 specific gene families (contained 150 160 genes) were found in the chromosomes and 34 77 specific gene families (contained 34 78 genes) in the megaplasmids from four strains. The average nucleotide identify (ANI) values between any two strains ranged from 99.05% 99.71%, and the proportion of the total base length of collinear blocks accounts for the total gene length of corresponding genome was all more than 93.82%. Then, we performed a search for genomic islands, prophage sequences, the gene clusters macromolecular secretion systems, type III secreted effectors and other virulence factors in these strains, which provided detailed comparison results of their presence and distinctive features compared to the reference strain GMI1000. Among them, the number and types of T2SS gene clusters were different in the four strains, among which RS650 included all five types. T4SS gene cluster of RS639 and RS647 were missed. In the T6SS gene cluster, several genes were inserted in the RS639, RS647, and RS650, and gene deletion was also detected in the RS642. A total of 78 kinds of type III secreted effectors were found, which included 52 core and 9 specific effectors in four strains. Conclusion This study not only provided the complete genomes of multiple R. pseudosolanacearum strains isolated from a new host, but also revealed the differences in their genomic levels through comparative genomics. Furthermore, these findings expand human knowledge about the range of hosts that Ralstonia can infect, and potentially contribute to exploring rules and factors of the genetic evolution and analyzing its pathogenic mechanism.