您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Kruppel-like factor 12a and 12b inhibit Cyvirus cyprinidallo3 replication by repressing viral ORF8 promoter activity

文献类型： 外文期刊

作者： Hu, Xinyue 1 ; Li, Yingying 1 ; Wang, Yingying 1 ; Zhang, Defeng 1 ; Liu, Donghai 1 ; Wang, Qing 1 ; Zheng, Shucheng 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Minist Agr & Rural Affairs, Guangdong Prov Key Lab Aquat Anim Immunol & Sustai, Key Lab Fishery Drug Dev,Pearl River Fisheries Res, Guangzhou 510380, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

3.Zhaoqing Branch Ctr Guangdong Lab Lingnan Modern A, Zhaoqing 526000, Peoples R China

关键词： Cyprinus carpio; Cyvirus cyprinidallo3; Cyprinid herpesvirus 3; Kruppel-like factor-12; ORF8

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：3.9； 五年影响因子：4.2 ）

ISSN： 1050-4648

年卷期： 2025 年 165 卷

页码：

收录情况： SCI

摘要： Cyvirus cyprinidallo3, also known as Cyprinid herpesvirus 3 (CyHV-3), is a highly infectious and lethal DNA virus causing considerable economic losses in the common carp and koi farming sectors. The pathogenesis caused by CyHV-3 is still poorly understood and effective control measures remain unavailable. Previously, we found that the immediate early (IE) gene ORF8 promoter of CyHV-3 exhibits the highest activity in the host cells. However, the transcriptional regulatory mechanism of the viral ORF8 promoter remains unknown. Herein, the potential transcription factors Kruppel-like factors (KLFs) 12a and 12b from common carp (Cyprinus carpio) targeting the ORF8 promoter were characterized. KLF12a and KLF12b were widely expressed in various tissues of common carp and the corresponding proteins are primarily localized to the nucleus of common carp brain cell line (CCB). Particularly, KLF12a and KLF12b overexpression significantly suppressed the ORF8 promoter activity and deletion of the ORF8 promoter region containing KLF12 binding sites resulted in a marked decrease in activity. Continuous deletion of the viral ORF8 promoter at 75 bp intervals further revealed that - 203 similar to-53 region is strongly required for the viral ORF8 promoter activity. More importantly, both KLF12a and KLF12b overexpression markedly reduced viral genome copies number, and virions in CCB cells. Taken together, Cyprinus carpio KLF12a and KLF12b inhibit CyHV-3 replication by repressing viral ORF8 promoter activity, which provides a foundation for further investigation into the transcriptional regulatory mechanisms of IE genes in CyHV3.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台