Parentage Determination of Chinese Hooksnout Carp (Opsariichthys bidens) Based on Microsatellite DNA Markers
文献类型： 外文期刊
作者： Ge, Jianhui 1 ; Xu, Xiaojun 1 ; Ren, Jindong 1 ; Shen, Xiaoming 3 ; Yu, Jin 4 ; Lou, Bao 1 ;
作者机构： 1.Inst Hydrobiol, Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China
2.Zhejiang Wanli Univ, Coll Biol & Environm Sci, Ningbo, Peoples R China
3.Deqing Haoyuan Aquat Seed Ind Co Ltd, Huzhou, Peoples R China
4.Agr & Rural Bur Longyou Cty, Longyou Aquaculture Dev Ctr, Quzhou, Peoples R China
期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.0； 五年影响因子：2.3 ）
ISSN： 1355-557X
年卷期： 2024 年 2024 卷
页码：
收录情况： SCI
摘要： In this study, a panel of 14 microsatellite markers was screened to determine the parentage of 144 progenies in 12 Chinese hooksnout carp (Opsariichthys bidens) full-sib families. The combined exclusion probabilities for 14 loci were 97.6% and 99.9%, respectively, when there was no available parent information or only one parent information was provided. Simulation analysis demonstrated that the power of five loci to exclude false parents exceeded 99.0%, while that of eight loci reached 99.9% based on allele frequency data obtained from full-sib families (168 individuals). Moreover, the cumulative assignment success rate reached 100% with known parental and filial information when utilizing 13 or more loci, even in cases where no parent information was available. The results showed that this set of microsatellite markers proved to be a reliable and efficient tool for parentage determination of O. bidens.
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