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Photo-controlled fluorescence "Double-Check" for human serum albumin and its applications

文献类型： 外文期刊

作者： Tang, Jun 1 ; Yang, Xiaopeng 1 ; Li, Jinsa 1 ; Zhang, Di 3 ; Wang, Yanliang 4 ; Ye, Yong 1 ;

作者机构： 1.Zhengzhou Univ, Green Catalysis Ctr, Zhengzhou 450001, Peoples R China

2.Zhengzhou Univ, Coll Chem, Zhengzhou 450001, Peoples R China

3.Henan Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Zhengzhou 450002, Peoples R China

4.Zhengzhou Univ, Peoples Hosp, Henan Prov Peoples Hosp, Dept Nephrol,Henan Prov Key Lab Kidney Dis & Immu, Zhengzhou 450003, Henan, Peoples R China

关键词： Photo-controlled; Fluorescence; Human serum albumin; Double-check; SO2

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：9.221； 五年影响因子：7.676 ）

ISSN：

年卷期： 2022 年 350 卷

页码：

收录情况： SCI

摘要： Photo-controlled method may improve the accuracy of fluorescent probes. Here, two new fluorescent probes HSP and MSP were designed based on naphthalimide and spiropyran. HSP exhibits a fluorescent emission at 540 nm after Human Serum Albumin (HSA) was added. Then with UV light irradiation, the spiropyran group of HSP is isomerized into the memcyanine, and the HSP/HSA hybrid displays red fluorescence emission at 610 nm, which demonstrats the probe have a unique fluorescence "double-check" mechanism to accurately detect HSA. As a comparison, a new probe MSP which structure similar to HSP was designed. Compared with HSP, the recognition effect of probe MSP on HSA is not obvious, which showed that the hydrophilic group hydroxyl displays an important influence on the response of the probe HSP to HSA. In addition, the HSP/HSA hybrid can also be used to detect SO2 effectively. Most importantly, we have successfully applied HSP/HSA hybrid to image SO2 in living cells.

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