文献类型： 外文期刊

作者： Ma, Ru ¹ ; Zhang, Zhenggui ² ; Wang, Jian ² ; Han, Yingchun ² ; Li, Ke ¹ ; Hou, Mengyao ¹ ; Lei, Yaping ² ; Xiong, Shiwu ² ; Yang, Beifang ² ; Zhi, Xiaoyu ² ; Jiao, Yahui ² ; Lin, Tao ⁴ ; Zhang, Shijie ¹ ; Li, Yabing ¹ ;

作者机构： 1.Zhengzhou Univ, Sch Agr Sci, State Key Lab Cotton Biobreeding & Integrated Util, Zhengzhou Res Base, Zhengzhou, Peoples R China

2.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biobreeding & Integrated Util, Anyang, Peoples R China

3.Zhengzhou Univ, Henan Key Lab Ion Beam Green Agr Bioengn, Zhengzhou, Peoples R China

4.Xinjiang Acad Agr Sci, Inst Cash Crops, Urumqi, Peoples R China

关键词： N2O emissions; NH3 volatilization; green manure; N fertilizer reduction; bacterial keystone taxa

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.5； 五年影响因子：5.2 ）

ISSN：

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： Integrating green manure with reduced nitrogen (N) fertilization is a promising strategy to mitigate N emissions in intensive cotton cultivation, however, the underlying mechanisms remain poorly understood. This study investigated the effects of three green manure incorporation patterns-no green manure (NG), Orychophragmus violaceus (OVG), and Vicia villosa (VVG)-combined with four N reduction levels (100, 50, 25%, and conventional) on gaseous N emissions (NH3 and N2O), soil physicochemical properties, and bacterial community characteristics using a cotton field experiment in the Yellow River Basin. Results showed that OVG incorporation with 25% N reduction (N2 treatment) significantly reduced total gaseous N emissions by 36.07% on average during the cotton growth period, reducing NH3 and N2O emissions by 13.31-54.11% and 32.25-68.77%, respectively, compared with N2 application without OVG. OVG application also increased the relative abundance of Proteobacteria (28.10%), enhanced heterogeneous selection in bacterial community assembly (200%), and increased the complexity of co-occurrence networks, compared with NG. Compared with conventional N fertilization (N3 treatment), >= 50% N reduction significantly lowered NH3 (>25.51%) and N2O (>32.76%) emissions, reduced the relative abundance of Acidobacteria (-20.23%), simplified co-occurrence networks, and increased homogeneous selection in bacterial assembly (50.00%). Integrating green manure with 25% N reduction substantially reduced gaseous N emissions, which was associated with the enhanced microbial biomass carbon (MBC) and facilitated recruitment of key bacterial taxa (e.g., Sphingosinicella, Azohydromonas, Phototrophicus) within the microbial co-occurrence network. These findings provide insight into how green manure application coupled with N reduction can mitigate gaseous N losses and reshape soil microbial ecology, offering a theoretical basis for sustainable nutrient management during cotton production.