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Genetically modified soybean lines exhibit less transcriptomic variation compared to natural varieties

文献类型： 外文期刊

作者： Long, Yan 1 ; Xu, Wentao 2 ; Liu, Caiyue 1 ; Dong, Mei 1 ; Liu, Weixiao 1 ; Pei, Xinwu 3 ; Li, Liang 1 ; Chen, Rui 1 ; Jin, Wujun 1 ;

作者机构： 1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing, Peoples R China

2.Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Coll Food Sci & Nutr Engn, Beijing, Peoples R China

3.Tianjin Acad Agr Sci, Biotechnol Res Inst, Tianjin, Peoples R China

4.Tianjin Acad Agr Sci, Biotechnol Res Inst, Tianjin 100081, Peoples R China

5.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

关键词： Differentially expressed genes; GM soybean lines; non-GM soybean lines; soybean seed tissues; transcriptomic analysis; >

期刊名称：GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN （ 影响因子：3.9； 五年影响因子：4.3 ）

ISSN： 2164-5698

年卷期： 2023 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Genetically modified (GM) soybeans provide a huge amount of food for human consumption and animal feed. However, the possibility of unexpected effects of transgenesis has increased food safety concerns. High-throughput sequencing profiling provides a potential approach to directly evaluate unintended effects caused by foreign genes. In this study, we performed transcriptomic analyses to evaluate differentially expressed genes (DEGs) in individual soybean tissues, including cotyledon (C), germ (G), hypocotyl (H), and radicle (R), instead of using the whole seed, from four GM and three non-GM soybean lines. A total of 3,351 DEGs were identified among the three non-GM soybean lines. When the GM lines were compared with their non-GM parents, 1,836 to 4,551 DEGs were identified. Furthermore, Gene Ontology (GO) analysis of the DEGs showed more abundant categories of GO items (199) among non-GM lines than between GM lines and the non-GM natural varieties (166). Results of Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that most KEGG pathways were the same for the two types of comparisons. The study successfully employed RNA sequencing to assess the differences in gene expression among four tissues of seven soybean varieties, and the results suggest that transgenes do not induce massive transcriptomic alterations in transgenic soybeans compared with those that exist among natural varieties. This work offers empirical evidence to investigate the genomic-level disparities induced by genetic modification in soybeans, specifically focusing on seed tissues.

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