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Identification and function analysis of bHLH genes in response to cold stress in sweetpotato

文献类型： 外文期刊

作者： Jin, Rong 1 ; Kim, Ho Soo 2 ; Yu, Tao 3 ; Zhang, Aijun 1 ; Yang, Yufeng 4 ; Liu, Ming 1 ; Yu, Wenhui 6 ; Zhao, Peng 1 ; Zhang, Qiangqiang 1 ; Cao, Qinghe 1 ; Kwak, Sang-Soo 2 ; Tang, Zhonghou 1 ;

作者机构： 1.Xuzhou Inst Agr Sci, Xuzhou Sweetpotato Res Ctr, Minist Agr, Key Lab Sweetpotato Biol & Genet Breeding, Xuzhou, Jiangsu, Peoples R China

2.Korea Res Inst Biosci & Biotechnol KRIBB, Plant Syst Engn Res Ctr, 125 Gwahak Ro, Daejeon 34141, South Korea

3.Liaoning Acad Agr Sci, Shenyang, Peoples R China

4.Zhengzhou Univ, Henan Acad Agr Sci, Postgrad T&R Base, Cereal Crops Res Inst, Zhengzhou, Peoples R China

5.Zhengzhou Univ, Sch Agr Sci, Zhengzhou, Peoples R China

6.Jiangsu Normal Univ, Sch Life Sci, Xuzhou, Jiangsu, Peoples R China

关键词： Sweetpotato; bHLH gene family; Cold stress; ICE1-Like gene

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.437； 五年影响因子：5.731 ）

ISSN： 0981-9428

年卷期： 2021 年 169 卷

页码：

收录情况： SCI

摘要： Basic/helix-loop-helix (bHLH) transcription factors are involved in various metabolic and physiological processes in plants. Sweetpotato (Ipomoea batatas (L.) Lam.) is an important crop in China but is highly susceptible to cold stress. However, little information on the bHLH gene family is available, and the function of this family in response to cold stress has not been revealed in sweetpotato. Here, 110 IbbHLHs were identified and classified into 17 categories based on phylogenetic relationships, conserved motifs and gene structure analyses. Except for 5 IbbHLHs, 90 IbbHLHs were putative E-box-binding proteins including 70 IbbHLHs belonging to G-box, whereas 15 IbbHLHs were putative non-E box-binding proteins based on DNA-binding analysis. In total, 37 pairs of segmental duplicated genes and 5 pairs of tandem duplication genes were identified within the IbbHLH gene family. The transcript level of 20 IbbHLHs was regulated by cold stress based on RNA-seq data, and 8 genes were selected for further quantitative real-time PCR (qRT-PCR) analysis. IbHLH8 and IbHLH92 are involved in network interaction with several genes related to abiotic and biotic stresses under cold treatment. IbbHLH79, an ICE1-like gene, was isolated and overexpressed in sweetpotato. The IbbHLH79 protein can activate the CBF (C-repeat Binding Factor) pathway, and IbbHLH79-overexpressing transgenic plants display enhanced cold tolerance. Taken together, these results provide valuable information on the IbbHLH gene family; in addition, several IbbHLHs may regulate cold stress, and the results suggest IbbHLH79 will be useful for molecular breeding of enhanced cold tolerance in sweetpotato.

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