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HutZ is required for efficient heme utilization and contributes to the pathogenicity of Avibacterium paragallinarum

文献类型： 外文期刊

作者： Huo, Caiyun 1 ; Jiao, Lijiao 1 ; Li, Guiping 1 ; Li, Donghai 1 ; Lin, Wutong 1 ; Sun, Yingjian 2 ; Sun, Huiling 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing Key Lab Prevent & Control Infect Dis Lives, Beijing, Peoples R China

2.Beijing Univ Agr, Anim Sci & Technol Coll, Beijing, Peoples R China

关键词： Avibacterium paragallinarum; HutZ; iron homeostasis; heme utilization; pathogenicity

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.7； 五年影响因子：5.9 ）

ISSN： 2165-0497

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Avibacterium paragallinarum is the pathogen that causes infectious coryza, a highly contagious respiratory disease that brings a serious threat to chickens. Heme utilization systems play an important role in bacterial adversity adaptation and pathogenicity, and our previous report found the presence of heme utilization (HutZ) in Av. paragallinarum. However, little is known about the function of HutZ in Av. paragallinarum. In this study, the HutZ mutant strain of Av. paragallinarum was successfully developed and identified by PCR and western blot analysis. Mutation of HutZ significantly retards bacterial growth under reduced iron conditions, indicating the regulatory role of HutZ on growth and iron acquisition. Notably, the HutZ mutant strain had slower growth than the wild-type strain when heme was provided as the sole source of iron; thus, HutZ is crucial for heme utilization in Av. paragallinarum. Moreover, the HutZ mutant strain exhibited a markedly compromised tolerance to acid stress compared to the wild-type strain. Pathogenicity analysis showed that mutation of HutZ significantly weakened the ability of bacteria to invade and reproduce in host macrophage cells in vitro. Furthermore, the HutZ mutation could significantly decrease the bacterial virulence in chickens, which displayed lower morbidity and milder clinical symptoms. Hence, this is the first study to demonstrate in-depth the essential roles of HutZ on iron homeostasis and pathogenesis of Av. paragallinarum, which provides novel insight into advances of new prophylactic vaccines against this kind of bacteria.

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[1]HutZ is required for efficient heme utilization and contributes to the pathogenicity of Avibacterium paragallinarum. Huo, Caiyun,Jiao, Lijiao,Li, Guiping,Li, Donghai,Lin, Wutong,Sun, Huiling,Jiao, Lijiao,Sun, Yingjian. 2023

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