中国水产科学研究院机构知识库

Molecular cloning, characterization, and expression analysis of a Broad-Complex homolog during development in the oriental river prawn Macrobrachium nipponense

收藏
分享
全文链接

文献类型：外文期刊

作者： Jiang, S. F. ¹ ; Zhang, Y. P.; Sun, S. M. ¹ ; Gong, Y. S. ¹ ; Xiong, Y. W. ¹ ; Qiao, H. ¹ ; Zhang, W. Y. ¹ ; Jin, S. B ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab Freshwater Fisheries & Germplasm Resource, Freshwater Fisheries Res Ctr, Minist Agr, Wuxi, Peoples R China

2.Chinese Acad Fishery Sci, Key

关键词： Macrobrachium nipponense;Broad-Complex;cDNA;Real-time quantitative polymerase chain reaction;Metamorphosis

期刊名称：GENETICS AND MOLECULAR RESEARCH （影响因子：0.764；五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 2 期

页码：

收录情况： SCI

摘要： Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors. In this study, we isolated the full-length cDNA of a BR-C homolog from the testes of the oriental river prawn (Macrobrachium nipponense), according to established expressed sequence tag information, using the rapid amplification of cDNA ends technique. The homolog was designated as MnBR-C. The full-length cDNA of MnBR-C contained a 1095-bp open reading frame encoding a precursor protein of 365 amino acid residues. Comparative and bioinformatic analyses revealed that MnBR-C exhibited a high degree of homology with BR-C proteins, and contained the BTB and Zf-H2C2-2 domains. Real-time quantitative polymerase chain reaction (qPCR) analysis revealed that the MnBR-C expression level varied significantly in the developing embryo, postembryonic larva, and adult tissue. Real-time qPCR showed that the MnBR-C gene was expressed in all of the tissues investigated, with the highest level of expression in the brain. In addition, MnBR-C was more abundantly expressed in the testes than in the ovaries.

相关文献

[1]牙鲆MHCⅡB基因多态性及其与鱼体抗病力关系的研究. 陈松林,张玉喜,中国海洋大学海洋生命学院. 2005

[2]Locus Mapping, Molecular Cloning, and Expression Analysis of rps6kb2, a Novel Metamorphosis-Related Gene in Chinese Tongue Sole (Cynoglossus semilaevis). Liu, Yang,Wei, Min,Zhao, Fazhen,Chen, Songlin,Liu, Yang,Wei, Min,Guo, Hua,Shao, Changwei,Meng, Liang,Xu, Wenteng,Wang, Na,Wang, Lei,Cui, Zhongkai,Yang, Yingming,Li, Yangzhen,Zhao, Fazhen,Chen, Songlin,Liu, Yang,Wei, Min,Guo, Hua,Shao, Changwei,Meng, Liang,Xu, Wenteng,Wang, Na,Wang, Lei,Cui, Zhongkai,Yang, Yingming,Li, Yangzhen,Zhao, Fazhen,Chen, Songlin,Guo, Hua,Power, Deborah M.,Cui, Zhongkai,Hou, Jilun,Mahboob, Shahid,Mahboob, Shahid.

[3]Reproductive performance of one-year-old Pacific abalone (Haliotis discus hannai) and its crossbreeding effect on offspring growth and survival. Li, Jiaqi,Fang, Jianguang,Mao, Yuze,Wang, Mingling,Liu, Xiao,Li, Jiaqi,Wang, Mingling,Liu, Xiao,Mao, Yuze,Fang, Jianguang,Liu, Guangmou,Bian, Dapeng. 2017

[4]First studies of embryonic and larval development of Coilia nasus (Engraulidae) under controlled conditions. Xu, Gangchun,Tang, Xue,Gu, Ruobo,Xu, Pao,Tang, Xue,Le, Guowei,Zhang, Chengxiang,Zheng, Jinliang. 2011

[5]Distortion of frontal bones results from cell apoptosis by the mechanical force from the up- migrating eye during metamorphosis in Paralichthys olivaceus. Sun, Mingyan,Wei, Fen,Li, Hui,Xu, Juan,Chen, Xinye,Gong, Xiaoling,Bao, Baolong,Tian, Yongsheng,Chen, Songlin.

[6]大菱鲆免疫球蛋白轻链IgL全长cDNA的分离、鉴定及表达分析. 王贤丽,张玉喜,孟亮,陈松林. 2010

[7]海蜇(Rhopilema esculentum)Wnt5基因:cDNA克隆、基因组结构与表达. 周春娅,朱玲,潘滢,谢明松,杨傲傲,陈四清,庄志猛. 2013

[8]草鱼天然抗性相关巨噬蛋白基因全长cDNA的克隆与表达分析. 范玉顶,徐进,罗晓松,周勇,肖艺,曾令兵. 2011

[9]真鲷肌肉生长抑素(MSTN)基因的克隆及表达分析. 叶寒青,陈松林. 2006

[10]牙鲆自然杀伤细胞增强因子(NKEF)全长CDNA的克隆及表达分析. 王志坚,陈松林,季相山. 2005

[11]缘管浒苔rbcL全长cDNA克隆与序列分析. 应成琦,尹顺吉,林森杰,沈轶,何培民. 2010

[12]海蜇Frizzled1基因的克隆及在无性繁殖中的表达. 潘滢,朱玲,周春娅,陈四清,庄志猛. 2018

[13]奥利亚罗非鱼性别相关基因DMRT1克隆及其分析. 王光花,吴婷婷,岳斌. 2006

[14]海蜇(Rhopilema esculentum)Notch基因的cDNA克隆和表达. 骆晓蕊,朱玲,周春娅,庄志猛,范艳君. 2017

[15]罗非鱼胰蛋白酶ⅠmRNA克隆与分析. 黎军胜,李建林,吴婷婷. 2006

[16]南方鲇生长激素完整cDNA的克隆及其DNA序列分析. 宋平,向筑,潘云峰,胡隐昌,胡珈瑞. 2002

[17]罗非鱼胰蛋白酶ⅡcDNA克隆与序列分析. 黎军胜,李建林,吴婷婷. 2005

[18]大菱鲆生长激素基因cDNA的克隆、序列分析及分子系统研究. 刘滨,臧晓南,刘顺梅,张学成,雷霁霖. 2008

[19]剑尾鱼热应激蛋白HSP70 cDNA片段的克隆与序列分析. 万文菊,王纪亭,石存斌,吴淑勤,柴同杰. 2007

[20]青虾(Macrobrachium nipponense)Kazal型丝氨酸蛋白酶抑制因子基因全长cDNA的克隆及表达. 张响,乔慧,傅洪拓,吴滟,龚永生,蒋速飞,熊贻伟. 2012

作者其他论文更多>>

Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase

作者：Rahman, N. M. A.;Fu, H. T.;Jin, S.;Bai, H. K.;Liang, G. X.;Gong, Y. S.;Wu, Y.;Fu, H. T.;Sun, S. M.;Qiao, H.;Jin, S.;Zhang, W. Y.;Xiong, Y. W.;Rahman, N. M. A.

关键词：Macrobrachium nipponense;Nitric oxide synthase;Cloning;Expression pattern;RT-qPCR;Post-larvae
Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense

作者：Rahman, N. M. A.;Fu, H.;Bai, H.;Jiang, F. W.;Liang, G.;Jiang, F. F.;Wu, Y.;Fu, H.;Qiao, H.;Jin, S.;Zhang, W.;Sun, S.;Gong, Y.;Xiong, Y.;Rahman, N. M. A.

关键词：Macrobrachium nipponense;Expression pattern;Feminization-1 homolog b (Fem1b);Crustacean;RT-qPCR;Post-larval
Genetic analysis of QTL for eye cross and eye diameter in common carp (Cyprinus carpio L.) using microsatellites and SNPs

作者：Jin, S. B.;Zhang, X. F.;Lu, J. G.;Jia, Z. Y.;Sun, X. W.;Jin, S. B.;Fu, H. T.

关键词：Mirror carp Cyprinus carpio L.;Quantitative trait loci;Eye cross and Eye diameter;Functional growth factor;Breeding;Correlation analysis
Characterization of the male-specific lethal 3 gene in the oriental river prawn, Macrobrachium nipponense

作者：Zhang, Y. P.;Sun, S. M.;Fu, H. T.;Ge, X. P.;Qiao, H.;Zhang, W. Y.;Xiong, Y. W.;Jiang, S. F.;Gong, Y. S.;Jin, S. B.

关键词：Developmental expression;Macrobrachium nipponense;Crustacean;Male-specific lethal 3
Comparative transcriptome analysis of testes and ovaries for the discovery of novel genes from Amur sturgeon (Acipenser schrenckii)

作者：Zhang, Y.;Dong, X. L.;Sun, D. J.;Jin, S. B.;Xi, Q. K.;Song, D.;Fu, H. T.

关键词：Acipenser schrenckii;Testes;Ovaries;Transcriptome;Reproduction
Identification of androgenic gland microRNA and their target genes to discover sex-related microRNA in the oriental river prawn, Macrobrachium nipponense

作者：Jin, S. B.;Fu, H. T.;Jin, S. B.;Fu, H. T.;Jiang, S. F.;Xiong, Y. W.;Qiao, H.;Zhang, W. Y.;Gong, Y. S.;Wu, Y.

关键词：Macrobrachium nipponense;Androgenic gland;microRNA;Targeted genes;Sex-determination
Genomic cloning, expression, and single nucleotide polymorphism association analysis of the insulin-like androgenic gland hormone gene in the oriental river prawn (Macrobrachium nipponense)

作者：Li, F. J.;Jiang, F. W.;Bai, H. K.;Fu, H. T.;Li, F. J.;Jin, S. B.;Sun, S. M.;Qiao, H.;Zhang, W. Y.

关键词：Oriental river prawn;IAG gene;Growth trait;Single nucleotide polymorphism

Molecular cloning, characterization, and expression analysis of a Broad-Complex homolog during development in the oriental river prawn Macrobrachium nipponense

作者其他论文 更多>>

Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase

Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense

Genetic analysis of QTL for eye cross and eye diameter in common carp (Cyprinus carpio L.) using microsatellites and SNPs

Characterization of the male-specific lethal 3 gene in the oriental river prawn, Macrobrachium nipponense

Comparative transcriptome analysis of testes and ovaries for the discovery of novel genes from Amur sturgeon (Acipenser schrenckii)

Identification of androgenic gland microRNA and their target genes to discover sex-related microRNA in the oriental river prawn, Macrobrachium nipponense

Genomic cloning, expression, and single nucleotide polymorphism association analysis of the insulin-like androgenic gland hormone gene in the oriental river prawn (Macrobrachium nipponense)

意 见 箱

作者其他论文更多>>

意见箱