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MicroRNA expression signature in the striated and smooth adductor muscles of Yesso scallop Patinopecten yessoensis

文献类型： 外文期刊

作者： Sun, Xiujun 1 ; Zhang, Tianshi 1 ; Li, Li 2 ; Tu, Kang 3 ; Yu, Tao 4 ; Wu, Biao 1 ; Zhou, Liqing 1 ; Tian, Jiteng 1 ; Liu, Zhihong 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Lab Marine Fisheries Sci & Food Prod Proc, Pilot Natl Lab Marine Sci & Technol Qingdao,Key La, Qingdao 266071, Peoples R China

2.Natl Oceanog Ctr, Qingdao 266104, Peoples R China

3.Putian Inst Aquaculture Sci Fujian Prov, Putian 351100, Peoples R China

4.Chinese Acad Fishery Sci, Changdao Enhancement & Expt Stn, Changdao, Peoples R China

5.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词： Scallop; Adductor muscle; MicroRNAs; Transcriptome; Antagomir

期刊名称：GENOMICS （ 影响因子：4.31； 五年影响因子：4.38 ）

ISSN： 0888-7543

年卷期： 2022 年 114 卷 4 期

页码：

收录情况： SCI

摘要： Increasing evidences point to the potential role of microRNAs (miRNAs) in muscle growth and development in animals. However, knowledge on the identity of miRNAs and their targets in molluscs remains largely unknown. Scallops have one large adductor muscle, composed of fast (striated) and slow (smooth) muscle types, which display great differences in muscle fibers, meat quality, cell types and molecular components. In the present study, we performed a comprehensive investigation of miRNA transcriptomes in fast and slow adductor muscles of Yesso scallop Patinopecten yessoensis. As a result, 47 differentially expressed miRNAs representing ten miRNA families were identified between the striated and smooth adductor muscles. The KEGG enrichment analysis of their target genes were mainly associated with amino acid metabolism, energy metabolism and glycan biosynthesis. The target genes of miR-133 and miR-71 were validated by the dual-luciferase reporter assays and miRNA antagomir treatment in vivo. The identification and functional validation of these different miRNAs in scallops will greatly help our understanding of miRNA regulatory mechanism that achieves the unique muscle phenotypes in scallops. The present findings provide the direct evidences for muscle-specific miRNAs involved in muscle growth and differentiation in molluscs.

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