文献类型： 外文期刊
作者： Song, Chengchuang 1 ; Fang, Xue 1 ; Yang, Zhaoxin 2 ; Wang, Qi 1 ; Meng, Fantong 1 ; Chen, Yaqi 1 ; Chen, Junhao 1 ; Zhao 1 ;
作者机构： 1.Jiangsu Normal Univ, Sch Life Sci, Inst Cellular & Mol Biol, Xuzhou 221116, Jiangsu, Peoples R China
2.Northwest A&F Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod Shaanxi Prov, Yangling 712100, Shaanxi, Peoples R China
3.Hainan Acad Agr Sci, Inst Anim Sci & Vet Med, Haikou 571100, Hainan, Peoples R China
关键词： miR-152; skeletal muscle; KLF6; proliferation
期刊名称：ANIMALS （ 影响因子：2.752； 五年影响因子：2.942 ）
ISSN： 2076-2615
年卷期： 2021 年 11 卷 10 期
页码：
收录情况： SCI
摘要： Simple Summary:& nbsp;Our study found that miR-152 functions in bovine myoblasts to inhibit proliferation. Our prediction and experimental verification revealed that Kruppel-Like Factor 6 (KLF6) is a direct target gene of miR-152. Therefore, miR-152 and its target gene KLF6 have certain effects on the development of bovine skeletal muscles.
Though miRNAs have been reported to regulate bovine myoblast proliferation, but many miRNAs still need to be further explored. Specifically, miR-152 is a highly expressed miRNA in cattle skeletal muscle tissues, but its function in skeletal muscle development is unknown. Herein, we aimed to investigate the role of miR-152 in regulating bovine myoblast proliferation. Functionally, RT-qPCR, Western blotting, EdU assay, and flow cytometry detection results showed that miR-152 inhibited bovine myoblast proliferation. Mechanistically, we demonstrated transcription factor KLF6 was a target gene of miR-152 by means of bioinformatics software prediction and dual-luciferase report analysis, which had been demonstrated to be favorable for myoblast proliferation. Collectively, our research suggested that miR-152 inhibits bovine myoblast proliferation via targeting KLF6.
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