文献类型： 外文期刊
作者： Song, Chengchuang 1 ; Fang, Xue 1 ; Yang, Zhaoxin 2 ; Wang, Qi 1 ; Meng, Fantong 1 ; Chen, Yaqi 1 ; Chen, Junhao 1 ; Zhao 1 ;
作者机构： 1.Jiangsu Normal Univ, Sch Life Sci, Inst Cellular & Mol Biol, Xuzhou 221116, Jiangsu, Peoples R China
2.Northwest A&F Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod Shaanxi Prov, Yangling 712100, Shaanxi, Peoples R China
3.Hainan Acad Agr Sci, Inst Anim Sci & Vet Med, Haikou 571100, Hainan, Peoples R China
关键词： miR-152; skeletal muscle; KLF6; proliferation
期刊名称：ANIMALS （ 影响因子：2.752； 五年影响因子：2.942 ）
ISSN： 2076-2615
年卷期： 2021 年 11 卷 10 期
页码：
收录情况： SCI
摘要： Simple Summary:& nbsp;Our study found that miR-152 functions in bovine myoblasts to inhibit proliferation. Our prediction and experimental verification revealed that Kruppel-Like Factor 6 (KLF6) is a direct target gene of miR-152. Therefore, miR-152 and its target gene KLF6 have certain effects on the development of bovine skeletal muscles.
Though miRNAs have been reported to regulate bovine myoblast proliferation, but many miRNAs still need to be further explored. Specifically, miR-152 is a highly expressed miRNA in cattle skeletal muscle tissues, but its function in skeletal muscle development is unknown. Herein, we aimed to investigate the role of miR-152 in regulating bovine myoblast proliferation. Functionally, RT-qPCR, Western blotting, EdU assay, and flow cytometry detection results showed that miR-152 inhibited bovine myoblast proliferation. Mechanistically, we demonstrated transcription factor KLF6 was a target gene of miR-152 by means of bioinformatics software prediction and dual-luciferase report analysis, which had been demonstrated to be favorable for myoblast proliferation. Collectively, our research suggested that miR-152 inhibits bovine myoblast proliferation via targeting KLF6.
- 相关文献
作者其他论文 更多>>
-
Molecular genetic identification of Wuzhishan ant chicken, a newly discovered resource in China
作者：Gu, Lihong;Zheng, Xinli;Chao, Zhe;He, Zhongchun;Wu, Fanghu;Zhang, Xiaohui;Shang, Yuanyuan;Chen, Yanmin;Liu, Xiangxiang;Mo, Shuhui;Chen, Youyi;Lu, Lizhi;Wei, Dong;Wei, Sheng;Xu, Tieshan
关键词：Wuzhishan ant chicken; genetic diversity; whole-genome resequencing; genetic structure; genetic resource
-
Integrated transcriptomics and metabolomics study of embryonic breast muscle of Jiaji ducks
作者：Gu, Lihong;Chen, Jile;Hu, Chengjun;Wang, Dingfa;Rong, Guang;Lv, Renlong;Xu, Tieshan;Gu, Lihong;Chen, Jile;Huan, Shuqian
关键词：Jiaji duck; Transcriptome; Metabolome; Breast muscle; Joint analysis
-
Supplementation of dietary areca nut extract modulates the growth performance, cecal microbiota composition, and immune function in Wenchang chickens
作者：Wang, Shiping;Dai, Jiahui;Dai, Wenting;Kang, Xiaoning;Wang, Hong;Jiang, Qicheng;Zheng, Xinli;He, Zhongchun;Gu, Lihong;Xu, Tieshan;Fu, An;Xing, Zengyang;Chen, Yiyong;Lu, Lizhi
关键词：Wenchang chickens; Areca catechu; growth performance; metabonomics; cecal microbiota; MHC-BF1; Daxx upon analysis
-
Young SINEs in pig genomes impact gene regulation, genetic diversity, and complex traits
作者：Zhao, Pengju;Yu, Dongyou;Han, Xinyan;Qian, Lichun;Wang, Zhengguang;Zhao, Pengju;Yu, Dongyou;Han, Xinyan;Qian, Lichun;Wang, Zhengguang;Gu, Lihong;Gao, Yahui;Liu, George E.;Pan, Zhangyuan;Zhou, Huaijun;Liu, Lei;Li, Xingzheng;Fang, Lingzhao
关键词：
-
Structural variation and introgression from wild populations in East Asian cattle genomes confer adaptation to local environment
作者：Xia, Xiaoting;Zhang, Fengwei;Li, Shuang;Luo, Xiaoyu;Dong, Zheng;Wang, Shikang;Jin, Liangliang;Dang, Ruihua;Huang, Yongzhen;Lan, Xianyong;Ren, Gang;Wang, Yu;Cheng, Haijian;Chen, Hong;Pang, Weijun;Lei, Chuzhao;Chen, Ningbo;Peng, Lixin;Pausch, Hubert;Leonard, Alexander S.;Crysnanto, Danang;Tong, Bin;Lenstra, Johannes A.;Han, Jianlin;Han, Jianlin;Li, Fuyong;Xu, Tieshan;Gu, Lihong;Gao, Yuanpeng;Ma, Zhijie;Cheng, Haijian;Ma, Yun
关键词：Structural variation; Genome assembly; Long-read sequencing; East Asian cattle
-
Effect of Heat Stress on Egg Production, Steroid Hormone Synthesis, and Related Gene Expression in Chicken Preovulatory Follicular Granulosa Cells
作者：Yan, Leyan;Hu, Mengdie;Lei, Mingming;Chen, Zhe;Zhu, Huanxi;Chen, Rong;Gu, Lihong
关键词：heat stress; granulosa cells; progesterone; estradiol; StAR; laying hens
-
Using Extract From the Stems and Leaves of Yizhi (Alpiniae oxyphyllae) as Feed Additive Increases Meat Quality and Intestinal Health in Ducks
作者：Ji, Fengjie;Rong, Guang;Hu, Chengjun;Sun, Weiping;Wang, Dingfa;Peng, Weiqi;Wu, Hongzhi;Zhou, Hanlin;Xu, Tieshan;Gu, Lihong;Lin, Dajie;Liu, Quanwei;Dai, Haofu
关键词：Yizhi (Alpinia oxyphylla); ducks; intestinal health; antibiotic alternatives; meat quality
