文献类型： 外文期刊

作者： Li, Xuefeng ¹ ; Xie, Lixue ² ; Chen, Xihong ¹ ; Chen, Jiangshan ¹ ; Shen, Jianguo ³ ; Gao, Fangluan ¹ ;

作者机构： 1.Fujian Agr & Forestry Univ, Inst Plant Virol, Fuzhou 350002, Peoples R China

2.Fujian Acad Agr Sci, Fruit Res Inst, Fuzhou 350013, Peoples R China

3.Technol Ctr Fuzhou Customs Dist, Fujian Key Lab Technol Res Inspect & Quarantine, Fuzhou 350001, Peoples R China

关键词： Turnip mosaic virus; Passion fruit; Potyvirus; Phylogenetic analysis

期刊名称：JOURNAL OF PLANT PATHOLOGY （ 影响因子：2.2； 五年影响因子：2.1 ）

ISSN： 1125-4653

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Contigs with sequence identities to turnip mosaic virus (TuMV) were detected by deep sequencing of the small RNAs extracted from a passion fruit (Passiflora edulis) plant showing virus-like symptoms in Fujian Province, China. The complete genome of the TuMV, designated TuMV-FJ here, was determined based on the sequences of these contigs and Sanger sequencing of RT-PCR and 5 & PRIME;/3' RACE amplicons. The TuMV-FJ genome consists of 9,833 nucleotides excluding the poly(A) tail. It contains an open reading frame encoding a polypeptide with 3164 amino acid residues. The polypeptide was predicted to be cleaved into ten functional proteins by viral proteases. Phylogenetic analysis indicated that TuMV-FJ is a member of a world-B phylogenetic group of TuMV. TuMV-FJ shared more than 94% nucleotide and amino acid sequence identities with its closest relative, namely BJ-B01, a TuMV isolate from Brassica oleracea. A RT-LAMP assay was developed to facilitate the molecular detection of TuMV-FJ. As far as we know, this is the first report of the complete genome sequence of TuMV infecting P. edulis. In addition, the RT-LAMP assay provides a rapid, robust and highly sensitive approach for the detection of TuMV in passion fruit.