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Insights into the developmental trajectories of zygotic embryo, embryogenic callus and somatic embryo in coconut by single-cell transcriptomic analysis

文献类型： 外文期刊

作者： Zhang, Dapeng 1 ; Li, Zhiying 1 ; Htwe, Yin Min 1 ; Shi, Peng 1 ; Wei, Xueke 1 ; Nie, Hao 1 ; Nin, Jingjing 1 ; Wu, Lihuan 4 ; Khan, Faiza Shafique 2 ; Yu, Qun 2 ; Batool, Saira 1 ; Wang, Yong 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Coconut Res Inst, Natl Key Lab Trop Crop Breeding, Wenchang 571339, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Sanya Res Inst, Hainan Key Lab Biosafety Monitoring & Mol Breeding, Sanya 572000, Hainan, Peoples R China

3.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan 430070, Hubei, Peoples R China

4.Guangzhou Gene Denovo Biotechnol Co Ltd, Guangzhou 510320, Guangdong, Peoples R China

关键词： scRNA-seq; Zygotic embryo; Embryogenic callus; Somatic embryogenesis; Coconut

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.9； 五年影响因子：6.0 ）

ISSN： 0926-6690

年卷期： 2024 年 212 卷

页码：

收录情况： SCI

摘要： Comprehensive utilization of coconut tissue culture in propagation and transformation is limited by the low efficiency of somatic embryogenesis. Understanding the potential mechanisms and developmental regulators (DRs) will facilitate the process. Here, we performed single-cell RNA sequencing (scRNA-seq) using protoplasts isolated from the zygotic embryo (ZE), embryogenic callus (EC) and somatic embryo (SE) of 'Hainan Tall' coconut. Cell types were identified with the assistance of known cell marker genes and further validated by RNA in situ hybridization. Differentiation trajectories from the ZE to SE transition were then constructed and identified potential DRs in the process. Notably, developmental initiation cells for both ZE and SE were identified as quiescent center-like cells (QC-L), while it was the dividing cell (DC) in EC. CnGRF12 (Growth Regulating Factor) was preferentially expressed in these developmental initiation cells, and its co-expression with CnGIF1 (GRFinteracting Factor) by transient transformation suggested that CnGRF12 has potential as a DR to accelerate transformation. Our comprehensive cell atlas provides single-cell-level insights into the developmental trajectories of ZE, EC and SE cells in coconut, and potential DRs investigated in this study could be useful for the future genotype-independent transformation and gene editing of coconut.

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