文献类型： 外文期刊

作者： Tang, Jing ¹ ; Feng, Yulong ¹ ; Zhang, Bo ¹ ; Wu, Yongbao ¹ ; Guo, Zhanbao ¹ ; Liang, Suyun ¹ ; Zhou, Zhengkui ¹ ; Xie, M ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Key Lab Anim Poultry Genet Breeding & Reprod, State Key Lab Anim Nutr,Minist Agr & Rural Affair, Beijing 100193, Peoples R China

2.Guizhou Acad Agr Sci, Guizhou Anim Husb & Vet Res Inst, Guiyang 550000, Guizhou, Peoples R China

关键词： Pantothenic acid deficiency; Intestinal hypofunction; Mucosal proteomics; Hypoglycemia; Actin cytoskeleton

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2021 年 22 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundPantothenic acid deficiency (PAD) results in growth depression and intestinal hypofunction of animals. However, the underlying molecular mechanisms remain to be elucidated. Mucosal proteome might reflect dietary influences on physiological processes.ResultsA total of 128 white Pekin ducks of one-day-old were randomly assigned to two groups, fed either a PAD or a pantothenic acid adequate (control, CON) diet. After a 16-day feeding period, two ducks from each replicate were sampled to measure plasma parameters, intestinal morphology, and mucosal proteome. Compared to the CON group, high mortality, growth retardation, fasting hypoglycemia, reduced plasma insulin, and oxidative stress were observed in the PAD group. Furthermore, PAD induced morphological alterations of the small intestine indicated by reduced villus height and villus surface area of duodenum, jejunum, and ileum. The duodenum mucosal proteome of ducks showed that 198 proteins were up-regulated and 223 proteins were down-regulated (>1.5-fold change) in the PAD group compared to those in the CON group. Selected proteins were confirmed by Western blotting. Pathway analysis of these proteins exhibited the suppression of glycolysis and gluconeogenesis, fatty acid beta oxidation, tricarboxylic acid cycle, oxidative phosphorylation, oxidative stress, and intestinal absorption in the PAD group, indicating impaired energy generation and abnormal intestinal absorption. We also show that nine out of eleven proteins involved in regulation of actin cytoskeleton were up-regulated by PAD, probably indicates reduced intestinal integrity.ConclusionPAD leads to growth depression and intestinal hypofunction of ducks, which are associated with impaired energy generation, abnormal intestinal absorption, and regulation of actin cytoskeleton processes. These findings provide insights into the mechanisms of intestinal hypofunction induced by PAD.