文献类型： 外文期刊

作者： Huang, Jingnan ¹ ; Wang, Tianbao ¹ ; Qiu, Yuanmei ¹ ; Hassanyar, Aqai Kalan ¹ ; Zhang, Zhaonan ¹ ; Sun, Qiaoling ¹ ; Ni, Xiaomin ⁵ ; Yu, Kejun ¹ ; Guo, Yongkang ¹ ; Yang, Changsheng ¹ ; Lu, Yang ¹ ; Nie, Hongyi ¹ ; Lin, Yan ¹ ; Li, Zhiguo ¹ ; Su, Songkun ¹ ;

作者机构： 1.Fujian Agr & Forestry Univ, Coll Anim Sci, Coll Bee Sci, Fuzhou 350002, Peoples R China

2.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Peoples R China

3.Univ Toulouse III Paul Sabatier, Lab Evolut & Divers Biol, UMR5174, CNRS, F-31062 Toulouse, France

4.Southwest Univ, Biol Sci Res Ctr, Chongqing 400715, Peoples R China

5.Univ Queensland, Fac Sci, Brisbane, Qld 4072, Australia

6.Heilongjiang Acad Agr Sci, Mudanjiang Branch, Mudanjiang 157041, Peoples R China

7.Fujian Agr & Forestry Univ, Acad Bee Sci, Fuzhou 350002, Peoples R China

关键词： Apis mellifera; olfactory performance; miRNAs; proboscis extension response (PER); brain

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2023 年 14 卷 5 期

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) play a vital role in the nerve regulation of honey bees (Apis mellifera). This study aims to investigate the differences in expression of miRNAs in a honey bee's brain for olfactory learning tasks and to explore their potential role in a honey bee's olfactory learning and memory. In this study, 12 day old honey bees with strong and weak olfactory performances were utilized to investigate the influence of miRNAs on olfactory learning behavior. The honey bee brains were dissected, and a small RNA-seq technique was used for high-throughput sequencing. The data analysis of the miRNA sequences revealed that 14 differentially expressed miRNAs (DEmiRNAs) between the two groups, strong (S) and weak (W), for olfactory performance in honey bees were identified, which included seven up-regulated and seven down-regulated. The qPCR verification results of the 14 miRNAs showed that four miRNAs (miR-184-3p, miR-276-3p, miR-87-3p, and miR-124-3p) were significantly associated with olfactory learning and memory. The target genes of these DEmiRNAs were subjected to the GO database annotation and KEGG pathway enrichment analyses. The functional annotation and pathway analysis showed that the neuroactive ligand-receptor interaction pathway, oxidative phosphorylation, biosynthesis of amino acids, pentose phosphate pathway, carbon metabolism, and terpenoid backbone biosynthesis may be a great important pathway related to olfactory learning and memory in honey bees. Our findings together further explained the relationship between olfactory performance and the brain function of honey bees at the molecular level and provides a basis for further study on miRNAs related to olfactory learning and memory in honey bees.