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Mutation of the PHYTOENE DESATURASE 3 gene causes yellowish-white petals in Brassica napus

文献类型： 外文期刊

作者： Zhao, Chuanji 1 ; Bin Safdar, Luqman 1 ; Xie, Meili 1 ; Shi, Meijuan 1 ; Dong, Zhixue 1 ; Yang, Li 1 ; Cheng, Xiaohui; 1 ;

作者机构： 1.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Hubei, Peoples R China

2.Huazhong Agr Univ, Natl Ctr Rapeseed Improvement, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China

3.Wageningen Univ & Res, Expt Plant Sci, Biosystemat Grp, Wageningen, Netherlands

4.Guizhou Acad Agr Sci, Guizhou Rapeseed Inst, Guiyang 550008, Guizhou, Peoples R China

关键词： Yellowish-white flower; BSA-seq; RNA-seq; BnaA08.PDS3; Carotenoid biosynthesis

期刊名称：CROP JOURNAL （ 影响因子：4.407； 五年影响因子：5.687 ）

ISSN： 2095-5421

年卷期： 2021 年 9 卷 5 期

页码：

收录情况： SCI

摘要： Oilseed rape (Brassica napus) with yellow flowers is an attractive ornamental landscape plant during the flowering period, and the development of different petal colors has become a breeding objective. Although yellowish flower color is a common variant observed in field-grown oilseed rape, the genetics behind this variation remains unclear. We obtained a yellowish-white flower (ywf) mutant from Zhongshuang 9 (ZS9) by ethyl methanesulfonate mutagenesis (EMS) treatment. Compared with ZS9, ywf exhibited a lower carotenoid content with a reduced and defective chromoplast ultrastructure in the petals. Genetic analysis revealed that the yellowish-white trait was controlled by a single recessive gene. Using bulked-segregant analysis sequencing (BSA-seq) and kompetitive allele-specific PCR (KASP), we performed map-based cloning of the ywf locus on chromosome A08 and found that ywf harbored a C-to-T substitution in the coding region, resulting in a premature translation termination. YWF, encoding phytoene desaturase 3 (PDS3), was highly expressed in oilseed rape petals and involved in carotenoid biosynthesis. Pathway enrichment analysis of the transcriptome profiles from ZS9 and ywf indicated the carotenoid biosynthesis pathway to be highly enriched. Further analyses of differentially expressed genes and carotenoid components revealed that the truncated BnaA08.PDS3 resulted in decreased carotenoid biosynthesis in the mutant. These results contribute to an understanding of the carotenoid biosynthesis pathway and manipulation of flower-color variation in B. napus. (C) 2020 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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