文献类型： 外文期刊
作者： Wang, Jinxing 1 ; Pu, Zigang 2 ; Zhang, Weiyao 1 ; Qu, Mengnan 1 ; Gao, Lusi 1 ; Pan, Wenjing 1 ; Sun, Yanan 1 ; Fu, Chunxu 1 ; Zhang, Ling 2 ; Huang, Mingkun 2 ; Hu, Yufang 2 ;
作者机构： 1.Heilongjiang Acad Agr Sci, Suihua Branch, Suihua 152052, Peoples R China
2.Jiangxi Prov & Chinese Acad Sci, Jiangxi Prov Key Lab Plant Germplasm Innovat & Gen, Lushan Bot Garden, Jiujiang 330022, Peoples R China
3.Heilongjiang Longke Seed Ind Grp Co Ltd, Harbin 150000, Peoples R China
关键词： Glycine max; GmJAG1; DAP-seq; motifs
期刊名称：PLANTS-BASEL （ 影响因子：4.0； 五年影响因子：4.4 ）
ISSN： 2223-7747
年卷期： 2024 年 13 卷 12 期
页码：
收录情况： SCI
摘要： Interaction between transcription factors (TFs) and motifs is essential for gene regulation and the subsequent phenotype formation. Soybean (Glycine max) JAGGEED 1 (GmJAG1) is a key TF that controls leaf shape, seed number and flower size. To understand the GmJAG1 binding motifs, in this study, we performed the GmJAG1 DNA affinity purification sequencing (DAP-seq) experiment, which is a powerful tool for the de novo motif prediction method. Two new significant GmJAG1 binding motifs were predicted and the EMSA experiments further verified the ability of GmJAG1 bound to these motifs. The potential binding sites in the downstream gene promoter were identified through motif scanning and a potential regulatory network mediated by GmJAG1 was constructed. These results served as important genomic resources for further understanding the regulatory mechanism of GmJAG1.
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