您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular cloning, characterization and expression analysis of multiple leptin genes in Jian carp (Cyprinus carpio var. Jian)

文献类型： 外文期刊

作者： Tang, Yongkai 1 ; Yu, Juhua 1 ; Li, Hongxia 2 ; Xu, Pao 1 ; Li, Jianlin 2 ; Ren, Hongtao 3 ;

作者机构： 1.Nanjing Agr Univ, Wuxi Fishery Coll, Wuxi 214081, Peoples R China

2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Peoples R China

3.Henan Univ Sci & Technol, Anim Sci & Technol Coll, Luoyang 471003, Peoples R C

关键词： Leptin;Cloning;Expression;Cyprinus carpio var. Jian

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

ISSN： 1096-4959

年卷期： 2013 年 166 卷 2 期

页码：

收录情况： SCI

摘要： Three leptin genes (jILEP-A1,jILEP-A2 and jILEP-B) were cloned and characterized in Jian carp (Cyprinus carpi var. jian), with a highly conserved organization encoded by two exons and one intron. jILEP-A1 and jILEP-A2 contained 93 and 102 bp introns, respectively, encoding a 171-amino acid protein. jILEP-B contained a 687 bp intron encoding a 168-aa protein. jILEP-A1 and jILEP-A2 shared 82.5% identity, but only 29.2% and 28.6% with jILEP-B, respectively. jILEP-Al, jILEP-A2, and jILEP-B were expressed in a wide range of tissues, but the expression pattern differed between juveniles and adults (and females and males). In juveniles, the expression of j 1LEP-A1 and jILEPB was higher than jILEP-A2, and was the highest in the liver and gonad. In females, jILEP-A1 and jILEP-A2 expression was the highest in the hypothalamus and liver, whereas jILEP-B mRNA was detected at low levels in all tissues. In males, jILEP-Al mRNA was expressed primarily in the hypothalamus, with only very low levels in the peripheral tissues. jILEP-A2 and j1LEP-B mRNA were primarily expressed in the muscle, hypothalamus, and liver. The expression of j/LEP-Al and jILEP-B mRNA was high in the ovary and testis, respectively. Our results suggest that leptins play an important physiological role in reproduction. (C) 2013 Elsevier Inc All rights reserved.

  • 相关文献

[1]Association study between single nucleotide polymorphisms in leptin and growth traits in Cyprinus carpio var. Jian. Tang, Y.,Li, H.,Li, J.,Yu, F.,Yu, J.. 2016

[2]Identification of housekeeping genes suitable for gene expression analysis in Jian carp (Cyprinus carpio var. jian). Tang, Yong-kai,Yu, Ju-hua,Xu, Pao,Li, Jian-lin,Li, Hong-xia,Tang, Yong-kai,Yu, Ju-hua,Xu, Pao,Ren, Hong-tao. 2012

[3]Molecular cloning of the black tiger shrimp (Penaeus monodon) elongation factor 2 (EF-2): sequence analysis and its expression on the ovarian maturation stage. Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Zhang, Dianchang,Huang, Jianhua,Guo, Yihui. 2008

[4]Cloning, characterization and expression analysis of a cold shock domain family member YB-1 in turbot Scophthalmus maximus. Zhang, Yu-Xi,Zhang, Bo,Chen, Song-Lin,Wang, Xian-Li,Yang, Chang-Geng. 2012

[5]Na+/K+-ATPase alpha-subunit in swimming crab Portunus trituberculatus: molecular cloning, characterization, and expression under low salinity stress. Han, Xiaolin,Liu, Ping,Gao, Baoquan,Wang, Haofeng,Duan, Yafei,Xu, Wenfei,Chen, Ping,Han, Xiaolin. 2015

[6]Transcript profiles of mitochondrial and cytoplasmic manganese superoxide dismutases in Exopalaemon carinicauda under ammonia stress. Ren Hai,Wu Jianhua,Ren Hai,Li Jian,Li Jitao,Liu Ping,Liang Zhongxiu. 2015

[7]Cloning of catalase and expression patterns of catalase and selenium-dependent glutathione peroxidase from Exopalaemon carinicauda in response to low salinity stress. Ren Hai,Ren Hai,Li Jian,Li Jitao,Ying Yu,Ge Hongxing,Li Dongli,Yu Tianji. 2015

[8]Identification, cloning and expression analysis of an alpha-CGTase produced by stain Y112. Hao, Jian-Hua,Sun, Jing-Jing,Liu, Jun-Zhong,Wang, Wei,Sun, Mi,Hao, Jian-Hua,Sun, Jing-Jing,Liu, Jun-Zhong,Wang, Wei,Sun, Mi,Huang, Li-Ping,Chen, Xiao-tong. 2017

[9]Molecular cloning and expression analysis of a F-type lectin gene from Japanese sea perch (Lateolabrax japonicus). Qiu, Lihua,Yang, Keng,Zhang, Hanhua,Li, Jianzhu,Zou, Falin,Jiang, Shigui,Lin, Liansheng. 2011

[10]Molecular cloning and expression of two HSP70 genes in the Wuchang bream (Megalobrama amblycephala Yih). Ming, Jianhua,Xie, Jun,Xu, Pao,Ge, Xianping,Liu, Bo,He, Yijin,Cheng, Yanfen,Zhou, Qunlan,Pan, Liangkun,Ming, Jianhua,Liu, Wenbin. 2010

[11]Cloning of a heat shock protein 90 (HSP90) gene and expression analysis in the ridgetail white prawn Exopalaemon carinicauda. Li, Jitao,Han, Junying,Chen, Ping,Chang, Zhigjang,He, Yuying,Liu, Ping,Wang, Qingyin,Li, Jian. 2012

[12]Molecular cloning and characterization of prophenoloxidase gene in swimming crab Portunus trituberculatus. Wang, Qingyin. 2010

[13]Molecular characterization and expression analysis of extracellular copper-zinc superoxide dismutase gene from swimming crab Portunus trituberculatus. Li, Jitao,Chen, Ping,Liu, Ping,Gao, Baoquan,Wang, Qingyin,Li, Jian.

[14]cDNA cloning, tissues, embryos and larvae expression analysis of Sox10 in half-smooth tongue-sole, Cynoglossus semilaevis. Deng, Si-Ping,Chen, Song-Lin,Deng, Si-Ping,Chen, Song-Lin,Deng, Si-Ping.

[15]Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda. Ge, Qianqian,Ge, Qianqian,Li, Jitao,Li, Jian,Zhao, Fazhen,Ren, Hai,Liang, Junping,Duan, Yafei.

[16]Molecular cloning and sexually dimorphic expression of DMRT4 gene in Oreochromis aureus. Gan, Xi,Luo, Yongju,Wu, Tingting,Cao, Jinling,Chen, Jianjie.

[17]Molecular cloning and expression analysis of cytochrome P450 3A gene in the turbot Scophthalmus maximus. Sun, Airong,Li, Jian,Chang, Zhiqiang,Li, Jitao,Wang, Qi,Sun, Airong,Huang, Jingzhou,Wang, Qi.

[18]Molecular cloning and mRNA expression of M-phase phosphoprotein 6 gene in black tiger shrimp (Penaeus monodon). Zhou, Jun,Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Huang, Jianhua,Yang, Lishi,Su, Tianfeng,Zhang, Dianchang,Zhou, Jun,Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Huang, Jianhua,Yang, Lishi,Su, Tianfeng,Zhang, Dianchang.

[19]半滑舌鳎两种leptin基因的体外重组表达和生物活性分析. 张雅星,王滨,柳学周,徐永江,史宝,姜燕,崔爱君,张正荣,孙冉冉. 2019

[20]蒙古红鲌肥胖基因cDNA的克隆与组织表达特异性研究. 潘庭双,龙良启,吴小平,罗晓松,邹世平. 2007

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台