miR-34a/Notch1b mediated autophagy and apoptosis contributes to oxidative stress amelioration by emodin in the intestine of teleost Megalobrama amblycephala
文献类型： 外文期刊
作者： Song, Changyou 1 ; Liu, Bo 1 ; Ge, Xianping 1 ; Li, Hongxia 1 ; Liu, Bo 1 ; Xu, Pao 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr & Rural Affairs, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi 214081, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Jiangsu, Peoples R China
3.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, 9 Shanshui East Rd, Wuxi 214081, Jiangsu, Peoples R China
关键词： Oxidative stress; miR-34a; Notch1b; Autophagy; Apoptosis; Emodin; Megalobrama amblycephala
期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）
ISSN： 0044-8486
年卷期： 2022 年 547 卷
页码：
收录情况： SCI
摘要： Stress stimulated reactive oxygen species (ROS) excessive accumulation is the principal cause of oxidative damage in aquatic animals. Severe oxidative stress could induce cell fate dysfunction, affect physiologic derangement, growth, and even survival. This study aims to investigate the ameliorative function of emodin in resist to oxidative stress via histomorphology, transcriptome, miRNAs, and mRNA-miRNA integrate analysis in the intestine of Megalobrama amblycephala. Morphologically, emodin alleviated oxidative stress induced autophagy in intestine. Differentially expressed miRNAs induced by oxidative stress and emodin were mainly involved in energy metabolism, oxidative homeostasis, cell apoptosis and cancerization signaling. Specifically, target prediction of miRNAs reveals Notch and apoptosis signaling were dysregulated. mRNA-miRNA integrated analysis reveals miR-34a and Notch1b were actively co-related in the regulatory network, and miR-34a was solidly confirmed as the negative regulator of Notch1b by targeting to the 3'-UTR. Transcriptional expression of key genes reveal emodin alleviates intestinal oxidative stress by activating miR-34a, and subsequently inhibit the expression of Notch receptors (DllA, Jag1b, and Jag2) and down-stream regulators (Hes1, Hey1, and FBW7). Meanwhile, apoptosis-related gene expression (Bax, Casp3, Casp8, Casp9, AIF1, AIF3, CytoC, and CDK4) were activated by oxidative stress, while were alleviated to the control level when treated with emodin. In conclusion, these results suggest that miR-34a/Notch1b is the potential therapeutic target for emodin to ameliorate oxidative stress in M. amblycephala.
- 相关文献
作者其他论文 更多>>
-
Taurochenodeoxycholic Acid Improves Growth, Physiology, Intestinal Microbiota, and Muscle Development in Red Swamp Crayfish (Procambarus clarkii)
作者：Xu, Xiaodi;Zheng, Xiaochuan;Song, Changyou;Liu, Xin;Zhou, Qunlan;Sun, Cunxin;Liu, Bo;Xu, Xiaodi;Zheng, Xiaochuan;Song, Changyou;Liu, Xin;Zhou, Qunlan;Sun, Cunxin;Liu, Bo;Wang, Aimin;Zhu, Aiming
关键词：TCDCA; muscle quality; protein deposition; gut microbiota;
Procambarus clarkii -
Beneficial effects of Haematococcus pluvialis on growth and hepatopancreas health status in crayfish (Procambarus clarkii) via remodeling the gut microbial metabolic functions
作者：Wang, Aimin;Hui, Wenjie;Tian, Hongyan;Zhang, Wuxiao;Xia, Silei;Liu, Fei;Yang, Wenping;Yu, Yebing;Hui, Wenjie;Deng, Xin;Deng, Xin;Liu, Bo;Shao, Peng;Zhu, Aimin;Shao, Peng
关键词：Procambarus clarkii; Haematococcus pluvialis; Growth; Hepatopancreas health; Gut microbiota
-
Effect of Replacement of Fish Meal With Cottonseed and Blood Meals on Growth, Serum Biochemistry, Body Composition, and Antioxidant Capacity in GIFT Tilapia Juveniles (Oreochromis niloticus)
作者：Liu, Xin;Liu, Bo;Zhou, Qunlan;Liu, Bo;Liu, Bo;Zhou, Qunlan;Sun, Cunxin;Zheng, Xiaochuan;Liu, Bo;Liu, Bo;Zhou, Qunlan;Sun, Cunxin;Zheng, Xiaochuan;Liu, Bo
关键词：blood chemistry; fish meal replacement; growth metrics; proximate composition analysis
-
Multi-omics and biochemical analyses provide insights into hepatic glucolipid metabolism in red tilapia ( Oreochromis spp.) under salinity stress
作者：Jiang, Bingjie;Tao, Yifan;Lu, Siqi;Hua, Jixiang;Li, Yan;Dong, Yalun;Xu, Pao;Qiang, Jun;Huang, Renshan;Xu, Pao;Qiang, Jun
关键词：Glucolipid metabolism; Multi-omics; Physiological response; Red tilapia; Salinity stress
-
The chromosomal-level genome assembly and annotation of pen shell Atrina pectinata
作者：Li, Zhuanzhuan;Chen, Xi;Ma, Peizhen;Liu, Zhihong;Sun, Xiujun;Zhou, Liqing;Wu, Biao;Li, Zhuanzhuan;Chen, Xi;Ma, Peizhen;Liu, Zhihong;Sun, Xiujun;Zhou, Liqing;Wu, Biao;Liu, Bo;Ren, Jianfeng;Zheng, Yanxin;Yu, Tao
关键词：
-
Genome-Wide Association Study and RNA-Seq Analysis Uncover Candidate Genes Controlling Growth Traits in Red Tilapia (Oreochromis spp.) Under Hyperosmotic Stress
作者：Jiang, Bingjie;Tao, Yifan;Lu, Siqi;Xu, Pao;Qiang, Jun;Liu, Kai;Tao, Wenjing;Badran, Mohamed Fekri;Saleh, Moustafa Hassan Lotfy;Aboueleila, Rahma Halim Mahmoud;Xu, Pao;Qiang, Jun;Liu, Kai
关键词：red tilapia; growth traits; RNA-seq; GWAS; candidate gene
-
Chromosome-scale genomes of ecologically and economically important rabbitfish Siganus guttatus and Siganus oramin
作者：Xian, Lin;Huang, Xiaolin;Liang, Yu;Ou-Yang, Yan;Guo, Huayang;Liu, Bo;Zhu, Kecheng;Liu, Baosuo;Zhang, Nan;Zhu, Tengfei;Zhang, Dianchang;Xian, Lin;Huang, Xiaolin;Liang, Yu;Ou-Yang, Yan;Guo, Huayang;Liu, Bo;Zhu, Kecheng;Liu, Baosuo;Zhang, Nan;Zhu, Tengfei;Zhang, Dianchang;Zhang, Dianchang;Xian, Lin;Sahu, Sunil Kumar;Li, Qiye;Sahu, Sunil Kumar;Li, Qiye;Huang, Xiaolin;Liang, Yu;Ou-Yang, Yan;Guo, Huayang;Liu, Bo;Zhu, Kecheng;Liu, Baosuo;Zhang, Nan;Zhu, Tengfei;Zhang, Dianchang;Li, Chao
关键词：Fish; Siganus guttatus; Siganus oramin; Genome; HiC
