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Enhanced metabolic resistance mechanism endows resistance to metamifop in Echinochloa crus-galli (L.) P. Beauv.

文献类型: 外文期刊

作者: Sun, Penglei 1 ; Niu, Liangliang 1 ; Lan, Xianmin 1 ; Yu, Haiyan 1 ; Cui, Hailan 1 ; Chen, Jingchao 1 ; Li, Xiangju 1 ;

作者机构: 1.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China

2.Guizhou Acad Agr Sci, Inst Plant Protect, Guiyang 550006, Peoples R China

关键词: Echinochloa crus-galli; Metamifop; Metabolic resistance; UPLC-MS/MS

期刊名称:PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY ( 影响因子:4.7; 五年影响因子:4.7 )

ISSN: 0048-3575

年卷期: 2023 年 197 卷

页码:

收录情况: SCI

摘要: Barnyardgrass (Echinochloa crus-galli (L.) P. Beauv.), one of the worst weeds in paddy fields in China, has been frequently reported evolving resistance to acetyl-CoA carboxylase (ACCase) inhibiting herbicides. However, in the previous research, more attention was paid to target-site resistance (TSR) mechanisms, the non-target-site resistance (NTSR) mechanisms have not been well-established. In this study, the potential mechanism of resis-tance in a metamifopresistant E. crus-galli collected from Kunshan city, Jiangsu Province, China was investi-gated. Dose-response assays showed that the phenotypic resistant population (JS-R) has evolved 4.3-fold resistance to metamifop compared with the phenotypic susceptible population (YN-S). The ACCase CT gene sequencing and relative ACCase gene expression levels studies showed that no mutations were detected in the ACCase CT gene in both YN-S and JS-R, and there was no significant difference in the relative ACCase gene expression between YN-S and JS-R. After the pre-processing of glutathione-S-transferase (GSTs) inhibitor NBD-Cl, the resistance level of JS-R to metamifop was reversed 18.73%. Furthermore, the GSTs activity of JS-R plants was significantly enhanced compared to that of YN-S plants. UPLC-MS/MS revealed that JS-R plants had faster metabolic rates to metamifop than YN-S plants. Meanwhile, the JS-R popultion exhibited resistant to cyhalofop-butyl and penoxsulam. In summary, this study presented a novel discovery regarding the global emergence of metabolic resistance to metamifop in E. crus-galli. The low-level resistance observed in the JS-R population was not found to be related to TSR but rather appeared to be primarily associated with the overexpression of genes in the GSTs metabolic enzyme superfamily.

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