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Tea and Citrus maxima complex induces apoptosis of human liver cancer cells via PI3K/AKT/mTOR pathway in vitro

文献类型： 外文期刊

作者： Wen, Shuai 1 ; An, Ran 2 ; Li, Dongli 2 ; Cao, Junxi 1 ; Li, Zhigang 1 ; Zhang, Wenji 1 ; Chen, Ruohong 1 ; Li, Qiuhua 1 ; Lai, Xingfei 1 ; Sun, Lingli 1 ; Sun, Shili 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Tea Res Inst, Guangdong Key Lab Tea Resources Innovat & Utilizat, Guangzhou 510640, Peoples R China

2.Wuyi Univ, Sch Biotechnol & Hlth Sci, Jiangmen 529020, Peoples R China

3.Int Healthcare Innovat Inst Jiangmen, Jiangmen 529040, Peoples R China

关键词： apoptosis; Citrus maxima cv; Tomentosa; liver cancer; signaling pathway; tea

期刊名称：CHINESE HERBAL MEDICINES （ 影响因子：3.8； 五年影响因子：2.7 ）

ISSN： 1674-6384

年卷期： 2022 年 14 卷 3 期

页码：

收录情况： SCI

摘要： Objective: In this study, black tea and Citrus maxima (BT-CM), yellow tea and C. maxima (YT-CM), green tea and C. maxima (GT-CM) as subjects, the active ingredient content and antioxidant activity of three tea and C. maxima (T-CM) were analyzed. The effects of three T-CMs on apoptosis of liver cells in vitro and its mechanism were further explored.Methods: National standard method and HPLC were used for active ingredient analysis. MTT, cell flow cytometry and Western blot were used to analyze the effects of three T-CMs on cell proliferation, apop-tosis, and its underlying molecular mechanism.Results: The content of tea polyphenols, free amino acids, ratio of polyphenols and amino acids, ester cat-echins, non-ester catechins and caffeine in YT-CM and GT-CM was significantly higher than that of BT -CM. The in vitro antioxidant capacity of YT-CM and GT-CM was also significantly stronger than that of BT-CM. Three T-CMs had the effects of inhibiting proliferation, arresting cell cycle and inducing apoptosis in HepG2 and Bel7402 cells, especially YT-CM and GT-CM. Western blot analysis showed three T-CMs activated PI3K/AKT/mTOR signaling pathway and regulated the expression levels of apoptosis-related proteins Bax, Bcl-2 and Caspase-3/9. YT-CM and GT-CM had better ability to change the signal pathway than BT-CM.Conclusion: In short, T-CMs, which combined different degrees of fermentation tea with C. maxima, were rich in nutrients and biologically active substances. T-CMs, especially YT-CM and GT-CM, are healthy drinks that help to prevent and treat liver cancer.(c) 2022 Tianjin Press of Chinese Herbal Medicines. Published by ELSEVIER B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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