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Protein-polysaccharide based double network microbeads improves stability of Bifidobacterium infantis ATCC 15697 in a gastro-Intestinal tract model (TIM-1)

文献类型： 外文期刊

作者： Khan, Wahab Ali 1 ; Butt, Masood Sadiq 2 ; Yasmin, Iqra 3 ; Wadood, Syed Abdul 1 ; Mahmood, Asif 5 ; Gad, Heba A. 6 ;

作者机构： 1.Univ Home Econ Lahore, Dept Food Sci & Technol, Lahore 54660, Pakistan

2.Univ Agr Faisalabad, Natl Inst Food Sci & Technol, Fac Food Nutr & Home Sci, Faisalabad 38040, Pakistan

3.Univ Chakwal, Dept Human Nutr & Dietet, Chakwal 48800, Pakistan

4.Minist Agr & Rural Affairs China, Inst Agroprod Safety & Nutr, Zhejiang Acad Agr Sci, Key Lab Informat Traceabil Agr Prod, Hangzhou 310021, Peoples R China

5.Univ Chakwal, Dept Pharm, Chakwal 48800, Pakistan

6.Ain Shams Univ, Fac Pharm, Dept Pharmaceut & Ind Pharm, Cairo 11566, Egypt

7.Batterjee Med Coll, Dept Pharmaceut Sci, Pharm Program, Jeddah 21442, Saudi Arabia

关键词： Encapsulation; Pea protein isolates; Bifidobacterium infantis; TIM-1; Ileal efflux; Gastric compartment; Survival rate

期刊名称：INTERNATIONAL JOURNAL OF PHARMACEUTICS （ 影响因子：5.8； 五年影响因子：5.8 ）

ISSN： 0378-5173

年卷期： 2024 年 652 卷

页码：

收录情况： SCI

摘要： Microencapsulation of probiotics is a main technique employed to improve cell survival in gastrointestinal tract (GIT). The present study investigated the impact of utilizing proteins i.e. Whey Protein Isolates (WPI), Pea Protein Isolates (PPI) or (WPI + PPI) complex based microbeads as encapsulating agents on the encapsulation efficiency (EE), diameter, morphology along with the survival and viability of Bifidobacterium infantis ATCC 15697. Results revealed that WPI + PPI combination had the highest EE% of the probiotics up to 94.09 % and the smoothest surface with less visible holes. WPI based beads revealed lower EE% and smaller size than PPI based ones. In addition, WPI based beads showed rough surface with visible signs of cracks, while PPI beads showed dense surfaces with pores and depressions. In contrast, the combination of the two proteins resulted in compact and smooth beads with less visible pores/wrinkles. The survival in gastrointestinal tract (GIT) was observed through TNO in -vitro gastrointestinal model (TIM-1) and results illustrated that all microbeads shrank in gastric phase while swelled in intestinal phase. In addition, in -vitro survival rate of free cells was very low in gastric phase (18.2 %) and intestinal phase (27.5 %). The free cells lost their viability after 28 days of storage (2.66 CFU/mL) with a maximum log reduction of 6.76, while all the encapsulated probiotic showed more than 106-7 log CFU/g viable cell. It was concluded that encapsulation improved the viability of probiotics in GIT and utilization of WPI + PPI in combination provided better protection to probiotics.

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