文献类型： 外文期刊

作者： Nong, Wenyan ¹ ; Yu, Yifei ¹ ; Aase-Remedios, Madeleine E. ² ; Xie, Yichun ¹ ; So, Wai Lok ¹ ; Li, Yiqian ¹ ; Wong, Cheuk Fung ¹ ; Baril, Toby ³ ; Law, Sean T. S. ¹ ; Lai, Sheung Yee ¹ ; Haimovitz, Jasmine ⁴ ; Swale, Thomas ⁴ ; Chen, Shan-Shan ⁵ ; Kai, Zhen-Peng ⁶ ; Sun, Xi ⁷ ; Wu, Zhongdao ⁷ ; Hayward, Alexander ³ ; Ferrier, David E. K. ² ; Hui, Jerome H. L. ¹ ;

作者机构： 1.Chinese Univ Hong Kong, Sch Life Sci, Simon FS Li Marine Sci Lab, State Key Lab Agrobiotechnol, Hong Kong, Peoples R China

2.Univ St Andrews, Scottish Oceans Inst, Gatty Marine Lab, Sch Biol, St Andrews, Fife, Scotland

3.Univ Exeter, Exeter, Devon, England

4.Dovetail Genom, Scotts Valley, CA USA

5.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, Shanghai, Peoples R China

6.Shanghai Inst Technol, Sch Chem & Environm Engn, Shanghai, Peoples R China

7.Sun Yat Sen Univ, Guangzhou, Guangdong, Peoples R China

期刊名称：GIGASCIENCE （ 影响因子：7.658； 五年影响因子：8.439 ）

ISSN： 2047-217X

年卷期： 2022 年 11 卷

页码：

收录情况： SCI

摘要： Background Schistosomiasis, or bilharzia, is a parasitic disease caused by trematode flatworms of the genus Schistosoma. Infection by Schistosoma mansoni in humans results when cercariae emerge into water from freshwater snails in the genus Biomphalaria and seek out and penetrate human skin. The snail Biomphalaria straminea is native to South America and is now also present in Central America and China, and represents a potential vector host for spreading schistosomiasis. To date, genomic information for the genus is restricted to the neotropical species Biomphalaria glabrata. This limits understanding of the biology and management of other schistosomiasis vectors, such as B. straminea. Findings Using a combination of Illumina short-read, 10X Genomics linked-read, and Hi-C sequencing data, our 1.005 Gb B. straminea genome assembly is of high contiguity, with a scaffold N50 of 25.3 Mb. Transcriptomes from adults were also obtained. Developmental homeobox genes, hormonal genes, and stress-response genes were identified, and repeat content was annotated (40.68% of genomic content). Comparisons with other mollusc genomes (including Gastropoda, Bivalvia, and Cephalopoda) revealed syntenic conservation, patterns of homeobox gene linkage indicative of evolutionary changes to gene clusters, expansion of heat shock protein genes, and the presence of sesquiterpenoid and cholesterol metabolic pathway genes in Gastropoda. In addition, hormone treatment together with RT-qPCR assay reveal a sesquiterpenoid hormone responsive system in B. straminea, illustrating that this renowned insect hormonal system is also present in the lophotrochozoan lineage. Conclusion This study provides the first genome assembly for the snail B. straminea and offers an unprecedented opportunity to address a variety of phenomena related to snail vectors of schistosomiasis, as well as evolutionary and genomics questions related to molluscs more widely.