F(0)ATP synthase b-chain of Litopenaeus vannamei involved in White Spot Syndrome Virus infection
文献类型: 外文期刊
作者: Li, Qian 1 ; Liu, Qing-hui 1 ; Huang, Jie 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Shanghai Ocean Univ, Shanghai 201306, Peoples R China
关键词: White Spot Syndrome Virus;VP292;F(0)ATP synthase b-chain;Interaction
期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )
ISSN: 0920-8569
年卷期: 2013 年 47 卷 1 期
页码:
收录情况: SCI
摘要: White Spot Syndrome Virus (WSSV) is one of the most common and distrous diseases for shrimp. In this study, we show that the protein VP292 that is a envelop protein of WSVV interacts with F(0)ATP synthase b-chain from Litopenaeus vannamei using far-western blot, ELISA, and indirect immunofluorescence analysis. Tissue distribution analysis of F(0)ATP synthase b-chain showed that it's transcription can be detected in muscle, hepatopancreas, intestine, hemocytes, lymphoid, and gills. Cellular localization of F(0)ATP synthase b-chain in shrimp hemocytes showed that F(0)ATP synthase b-chain was primarily located in the cytoplasm of hemocytes. The transcription levels of F(0)ATP synthase b-chain were significantly upregulated in intestine, hepatopancreas, hemocytes, and gills of WSSV-infected shrimp at 12 h after infection. Far-western, ELISA, and indirect immunefluorescence assay indicated that F(0)ATP synthase b-chain interacted with VP292. In the in vivo neutralization experiment, F(0)ATP synthase b-chain attained 18 % protection rate of the shrimp challenged by WSSV. To the best of our knowledge, this is the first report to show that F(0)ATP synthase b-chain is involved in WSSV infection.
- 相关文献
作者其他论文 更多>>
-
Coinfection with infectious precocity virus and Decapod iridescent virus 1 in farmed giant freshwater prawn ( Macrobrachium rosenbergii )
作者:Wang, Guohao;Guo, Xiaomeng;Zhou, Chengyan;Huang, Jie;Yao, Jiayun;Wang, Guohao;Guo, Xiaomeng;Zhou, Chengyan;Lou, Haoyu;Li, Xuan;Dong, Xuan;Huang, Jie;Li, Xuan;Dong, Xuan;Dong, Xuan;Yang, Guoliang;Huang, Jie;Huang, Jie;Yao, Jiayun;Dong, Xuan
关键词:IPV; DIV1; Coinfection; Macrobrachium rosenbergii
-
Development of a Melting Curve-Based Triple Eva Green Real-Time PCR Assay for Simultaneous Detection of Three Shrimp Pathogens
作者:Dong, Xuan;Chen, Yujin;Lou, Haoyu;Wang, Guohao;Zhou, Chengyan;Wang, Liying;Li, Xuan;Luo, Jingfei;Huang, Jie;Dong, Xuan;Huang, Jie;Chen, Yujin;Huang, Jie
关键词:aquaculture; shrimp pathogens; Eva Green; real-time PCR; detection
-
Establishment of a real-time PCR for the detection of decapod iridescent virus 1 (DIV1)
作者:Guo, Xiao-Meng;Xing, Jing-Yi;Qiu, Liang;Zhang, Qing-Li;Huang, Jie;Guo, Xiao-Meng;Zhang, Qing-Li;Huang, Jie;Xing, Jing-Yi;Li, Anqi;Zhang, Qing-Li;Huang, Jie;Qiu, Liang
关键词:Cherax quadricarinatus iridovirus; decapod iridescent virus 1; molecular diagnosis; real-time PCR; Shrimp hemocyte iridescent virus
-
Development of Monoclonal Antibody against PirB and Establishment of a Colloidal Gold Immunochromatographic Assay for the Rapid Detection of AHPND-Causing Vibrio
作者:Dong, Xuan;Xie, Jingmei;Wang, Liying;Li, Xuan;Lou, Haoyu;Wang, Guohao;Huang, Jie;Dong, Xuan
关键词:AHPND; Vibrio; PirB; monoclonal antibody; colloidal gold immunochromatographic assay
-
Is Artemia susceptible to the microsporidium Ecytonucleospora hepatopenaei (EHP) infection?
作者:Guo, Xiao-Meng;Huang, Jie;Guo, Xiao-Meng;Gao, Wen;Chen, Xing;Wang, Hai-Liang;Zhao, Ruo-Heng;Xie, Guo-Si;Huang, Jie;Guo, Xiao-Meng;Gao, Wen;Chen, Xing;Wang, Hai-Liang;Zhao, Ruo-Heng;Xie, Guo-Si;Huang, Jie;Huang, Jie
关键词:Artemia franciscana; Ecytonucleospora hepatopenaei; Susceptibility
-
Meta-Transcriptomic Analysis Reveals Novel RNA Viruses in Polychaetes Perinereis
作者:Luo, Jingfei;Zhou, Chengyan;Wang, Guohao;Huang, Jie;Dong, Xuan;Luo, Jingfei;Zhang, Fan;Zhou, Chengyan;Meng, Fanzeng;Wang, Guohao;Qiu, Liang;Huang, Jie;Dong, Xuan;Shi, Weifeng;Dong, Xuan
关键词:RNA viruses; Perinereis; meta-transcriptomic; virome; aquaculture
-
Macrophthalmus (Macrophthalmus) abbreviatus Manning & Holthuis, 1981, a new natural host for Hematodinium perezi infection
作者:Liu, Zhengmin;Xie, Guosi;Wan, Xiaoyuan;Li, Ang;Zhou, Liqing;Shi, Chengyin;Zhang, Qingli;Huang, Jie;Liu, Zhengmin;Xie, Guosi;Wang, Hailiang;Wan, Xiaoyuan;Shi, Chengyin;Zhang, Qingli;Huang, Jie;Liu, Zhengmin;Xie, Guosi;Wan, Xiaoyuan;Shi, Chengyin;Zhang, Qingli;Huang, Jie;Li, Xinshu;Huang, Jie
关键词:parasitic dinoflagellates; Hematodinium sp.; Hematodinium perezi; Macrophthalmus abbreviatus; host