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F(0)ATP synthase b-chain of Litopenaeus vannamei involved in White Spot Syndrome Virus infection

文献类型: 外文期刊

作者: Li, Qian 1 ; Liu, Qing-hui 1 ; Huang, Jie 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Shanghai Ocean Univ, Shanghai 201306, Peoples R China

关键词: White Spot Syndrome Virus;VP292;F(0)ATP synthase b-chain;Interaction

期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )

ISSN: 0920-8569

年卷期: 2013 年 47 卷 1 期

页码:

收录情况: SCI

摘要: White Spot Syndrome Virus (WSSV) is one of the most common and distrous diseases for shrimp. In this study, we show that the protein VP292 that is a envelop protein of WSVV interacts with F(0)ATP synthase b-chain from Litopenaeus vannamei using far-western blot, ELISA, and indirect immunofluorescence analysis. Tissue distribution analysis of F(0)ATP synthase b-chain showed that it's transcription can be detected in muscle, hepatopancreas, intestine, hemocytes, lymphoid, and gills. Cellular localization of F(0)ATP synthase b-chain in shrimp hemocytes showed that F(0)ATP synthase b-chain was primarily located in the cytoplasm of hemocytes. The transcription levels of F(0)ATP synthase b-chain were significantly upregulated in intestine, hepatopancreas, hemocytes, and gills of WSSV-infected shrimp at 12 h after infection. Far-western, ELISA, and indirect immunefluorescence assay indicated that F(0)ATP synthase b-chain interacted with VP292. In the in vivo neutralization experiment, F(0)ATP synthase b-chain attained 18 % protection rate of the shrimp challenged by WSSV. To the best of our knowledge, this is the first report to show that F(0)ATP synthase b-chain is involved in WSSV infection.

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