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SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis

文献类型： 外文期刊

作者： Zou, Xiaoyan 1 ; Huang, Rui 1 ; Wang, Linjie 1 ; Wang, Guihua 3 ; Miao, Ye 1 ; Rao, Idupulapati 4 ; Liu, Guodao 1 ; Chen, Zhijian 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Crop Genet Resources, Key Lab Trop Crops Germplasm Resources Genet Impro, Haikou, Peoples R China

2.Hainan Univ, Coll Trop Crops, Haikou, Peoples R China

3.Chinese Acad Trop Agr Sci, Rubber Res Inst, Haikou, Peoples R China

4.Alliance Biovers Int & Int Ctr Trop Agr, Crops Nutr & Hlth, Cali, Colombia

关键词： nramp; trace metal; metal homeostasis; gene expression; subcellular localization

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Transporters belonging to the natural resistance-associated macrophage protein (Nramp) family play important roles in metal uptake and homeostasis. Although Nramp members have been functionally characterized in plants, the role of Nramp in the important tropical forage legume Stylosanthes guianensis (stylo) is largely unknown. This study aimed to determine the responses of Nramp genes to metal stresses and investigate its metal transport activity in stylo. Five SgNramp genes were identified from stylo. Expression analysis showed that SgNramp genes exhibited tissue preferential expressions and diverse responses to metal stresses, especially for manganese (Mn), suggesting the involvement of SgNramps in the response of stylo to metal stresses. Of the five SgNramps, SgNramp1 displayed the highest expression in stylo roots. A close correlation between SgNramp1 expression and root Mn concentration was observed among nine stylo cultivars under Mn limited condition. The higher expression of SgNramp1 was correlated with a high Mn uptake in stylo. Subsequent subcellular localization analysis showed that SgNramp1 was localized to the plasma membrane. Furthermore, heterologous expression of SgNramp1 complemented the phenotype of the Mn uptake-defective yeast (Saccharomyces cerevisiae) mutant Delta smf1. Mn concentration in the yeast cells expressing SgNramp1 was higher than that of the empty vector control, suggesting the transport activity of SgNramp1 for Mn in yeast. Taken together, this study reveals that SgNramp1 is a plasma membrane-localized transporter responsible for Mn uptake in stylo.

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