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Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis

文献类型： 外文期刊

作者： Shao, Chang-Sheng 1 ; Feng, Na 3 ; Zhou, Shuai 3 ; Zheng, Xin-Xin 1 ; Wang, Peng 1 ; Zhang, Jing-Song 3 ; Huang, Qing 1 ;

作者机构： 1.Chinese Acad Sci, Hefei Inst Phys Sci, CAS Key Lab High Magnet Field & Ion Beam Phys Bio, Hefei 230031, Peoples R China

2.Univ Sci & Technol China, Sci Isl Branch, Grad Sch, Hefei 230026, Peoples R China

3.Minist Agr, Key Lab Edible Fungi Resources & Utilizat South, Beijing, Peoples R China

4.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Inst Edible Fungi, Shanghai 201403, Peoples R China

关键词： ganoderic acid T (GAT); triterpene; apoptosis; necroptosis; radiosensitivity; ionizing radiation

期刊名称：TOXICOLOGY RESEARCH （ 影响因子：3.524； 五年影响因子：2.865 ）

ISSN： 2045-452X

年卷期： 2021 年 10 卷 3 期

页码：

收录情况： SCI

摘要： The use of natural substances derived from traditional Chinese medicine and natural plants as safe radiosensitizing adjuvants is a new trend for cancer radiotherapy. Ganoderma lucidum has been used as a traditional Chinese medicine with a history of more than 2000 years. Ganoderic acid T (GAT) is a typical triterpene of G. lucidum, which has strong cytotoxicity to cancer cells, but whether it has radiation sensitization effect has not been explored. In this work, we treated the HeLa cells with different concentrations of GAT before exposure to gamma-ray radiation and investigated its influence on the radiosensitivity. The cell viability, apoptosis rate, necoptosis rate, intracellular ATP level, cell cycle, the amount of H2AX and 53BP1, reactive oxygen species, and mitochondrial membrane potential were examined. Apoptotic, necroptotic, and autophagic biomarker proteins, including caspase 8, cytochrome c, caspase 3, RIPK, MLKL, P62, and LC3, were analyzed. As a result, we confirmed that with treatment of GAT, the gamma-ray radiation induced both apoptosis and necroptosis in HeLa cells, and with increase of GAT, the percentage ratio of necroptosis was increased. The involved pathways and mechanisms were also explored and discussed.

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