您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Heat enhances the inhibitory effect of lipopolysaccharide on duck granulosa cell proliferation and steroid biosynthesis in vitro

文献类型： 外文期刊

作者： Luo, Pei 1 ; Huang, Xue-bing 1 ; Zhan, Xiao-zhi 1 ; Yang, Chen 1 ; Deng, Zhi-chao 1 ; Zhang, Chen 1 ; Fu, Xin-liang 1 ; Tian, Yun-bo 1 ; Huang, Yun-mao 1 ; Liu, Wen-jun 1 ;

作者机构： 1.Zhong kai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou, Peoples R China

2.Guangdong Prov Key Lab Waterfowl Hlth Breeding, Guangzhou, Peoples R China

3.Guangdong Acad Agr Sci, Inst Anim Sci, State Key Lab Livestock & Poultry Breeding, Guangzhou, Peoples R China

4.Zhong kai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou 510225, Peoples R China

关键词： granulosa cells; heat treatment; lipopolysaccharide; proliferation; secretion

期刊名称：ANIMAL SCIENCE JOURNAL （ 影响因子：2.0； 五年影响因子：2.1 ）

ISSN： 1344-3941

年卷期： 2023 年 94 卷 1 期

页码：

收录情况： SCI

摘要： Lipopolysaccharide (LPS) reduces the reproductive performance of laying ducks, especially during the hot summer months. To study the underlying mechanisms, we investigated the effects of different LPS concentrations and heat on duck granulosa cell (GC) proliferation and steroid biosynthesis in vitro. We investigated GC proliferation, secretion, and activation of the MAPK pathway. The cell cycle results showed that LPS treatment alone did not significantly affect cell proliferation, whereas the mRNA expression levels of IGF2, IGFBP2, and CyclinD1 were downregulated and p27kip1 was significantly upregulated after 2000 ng/mL LPS treatment when compared to untreated cells. In steroid hormone synthesis, although LPS increased the expression of most steroid biosynthesis genes, it inhibited the expression of CYP11A1 at high LPS concentrations. High temperatures enhanced the inhibitory effect of LPS on the expression of proliferation-promoting genes. Heat significantly reduced CYP11A1 and CYP19A1 expression. In addition, the phosphorylation of P38 was significantly upregulated by high temperatures combined with LPS, whereas the phosphorylation of ERK1/2 and JNK was downregulated. The relative protein expression of Bax/BCL-2 was upregulated at high temperatures in combination with LPS. Heat treatment enhanced the inhibitory effects of LPS on the proliferation and hormone biosynthesis of duck GCs in vitro.

  • 相关文献

[1]Heat enhances the inhibitory effect of lipopolysaccharide on duck granulosa cell proliferation and steroid biosynthesis in vitro. Luo, Pei,Huang, Xue-bing,Zhan, Xiao-zhi,Yang, Chen,Deng, Zhi-chao,Zhang, Chen,Fu, Xin-liang,Tian, Yun-bo,Huang, Yun-mao,Liu, Wen-jun,Luo, Pei,Huang, Xue-bing,Zhan, Xiao-zhi,Yang, Chen,Deng, Zhi-chao,Zhang, Chen,Fu, Xin-liang,Tian, Yun-bo,Huang, Yun-mao,Liu, Wen-jun,Huang, Xue-bing,Huang, Xue-bing,Huang, Yun-mao,Liu, Wen-jun. 2023

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台