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Isolation and characterization of forty-two polymorphic simple sequence repeat markers from Mastacembelus armatus (Symbranchiformes: Mastacembelidae)

文献类型: 外文期刊

作者: Luo, Laifu 1 ; Ji, Liqin 2 ; Wu, Congcong 2 ; Li, Wei 2 ; Liu, Xiaoli 2 ; Hong, Xiaoyou 2 ; Zhu, Xinping 2 ; Ni, Yijun 3 ; Li, Jiansong 3 ; Chen, Chen 2 ; Zhang, Jiping 1 ;

作者机构: 1.Foshan Univ, Sch Life Sci & Engn, Foshan 528225, Peoples R China

2.Chinese Acad Fishery Sci, Key Lab Trop & Subtrop Fishery Resources Applicat, Minist Agr & Rural Affairs, Pearl River Fisheries Res Inst, Guangzhou 510380, Peoples R China

3.Huizhou Caixing Ind Co Ltd, Huizhou 516029, Peoples R China

关键词: Mastacembelus armatus; Genetic conservation; Microsatellite

期刊名称:CONSERVATION GENETICS RESOURCES ( 影响因子:0.9; 五年影响因子:0.9 )

ISSN: 1877-7252

年卷期: 2024 年 16 卷 4 期

页码:

收录情况: SCI

摘要: A total of 42 polymorphic simple sequence repeat (SSR) markers were developed and characterized from genome sequences for the "zig-zag eel", Mastacembelus armatus, to safeguard its natural resources. Polymorphisms of these SSR markers were evaluated in a natural population of 35 individuals. The number of alleles per locus ranged from two to fourteen. The observed heterozygosity and expected heterozygosity varied from 0.031 to 0.813, and from 0.091 to 0.852, respectively. Three SSR loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These loci have been characterized for the first time and will be useful for genetic conservation and marker-assisted breeding of M. armatus.

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