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Engineering the key domains of starch synthases and branching enzyme to balance the amylose increase and yield loss in maize kernels

文献类型： 外文期刊

作者： Wang, Zhengqi 1 ; Hu, Shuting 1 ; Zhuang, Junhong 4 ; Zhang, Xuan 1 ; Ge, Xingyu 1 ; Xu, Sixuan 1 ; Zhao, Xiaoming 4 ; Tang, Huaijun 5 ; Wang, Xiqing 4 ; Li, Jiansheng 1 ; Yang, Xiaohong 1 ;

作者机构： 1.China Agr Univ, State Key Lab Plant Environm Resilience, Beijing, Peoples R China

2.China Agr Univ, Natl Maize Improvement Ctr China, Beijing, Peoples R China

3.Sanya Inst China Agr Univ, Sanya, Peoples R China

4.China Agr Univ, Ctr Crop Funct Genom & Mol Breeding, Beijing, Peoples R China

5.Xinjiang Acad Agr Sci, Inst Grain Crops, Urumqi, Peoples R China

6.China Agr Univ, Frontiers Sci Ctr Mol Design Breeding, Beijing 100094, Peoples R China

关键词： Maize; Gene editing; SSIIa; SBEIIb; High-amylose starch

期刊名称：MOLECULAR BREEDING （ 影响因子：3.0； 五年影响因子：3.0 ）

ISSN： 1380-3743

年卷期： 2025 年 45 卷 4 期

页码：

收录情况： SCI

摘要： Amylose content and resistant starch (RS) are of great importance due to their multiple functionalities in the food and pharmaceutical industries and their benefits for human health. However, breeding high-amylose maize remains challenging because of the trade-off between amylose content and yield loss. Here, we report targeted mutagenesis of the key domains of starch synthases and branching enzyme including SSIIa, SSIII and SBEIIb via a CRISPR-Cas9 technology; this generated 15, 21, and 14 novel alleles, respectively, in the maize inbred line LH244. Except for ssIII mutants, the ssIIa and sbeIIb mutants had significantly greater apparent amylose content (AAC) and RS content compared with wild-type kernels. Although most mutants had reduced hundred-kernel weight (HKW) relative to wild-type plants, some mutants had only a small HKW reduction. Investigation of six representative mutants revealed that mutants of ssIIa and sbeIIb with higher AAC and RS content were accompanied with reduced starch content viscosity, increased content of reducing sugars and soluble sugars, and yet no apparent trade-off with agronomic traits. These findings offer a promising path for high-amylose maize breeding, accelerating the development of germplasms with enhanced RS content for the benefit of both global health and industry.

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