您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Establishment of an efficient regeneration and Agrobacterium transformation system in mature embryos of calla lily (Zantedeschia spp.)

文献类型： 外文期刊

作者： Sun, Xuan 1 ; Wang, Yi 2 ; Yang, Tuo 3 ; Wang, Xue 2 ; Wang, Huanxiao 1 ; Wang, Di 1 ; Liu, Hongyan 1 ; Wang, Xian 2 ; Zhang, Guojun 1 ; Wei, Zunzheng 2 ;

作者机构： 1.Hebei Normal Univ Sci & Technol, Coll Hort Sci & Technol, Hebei Key Lab Hort Germplasm Excavat & Innovat Uti, Qinhuangdao, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Grassland Flowers & Ecol, Beijing, Peoples R China

3.China Agr Univ, Coll Hort, Beijing, Peoples R China

4.Hebei Higher Inst Applicat Technol Res, Dev Ctr Hort Plant Biol Breeding, Qinhuangdao, Peoples R China

关键词： calla lily; regeneration system; genetic transformation; CNTs; seed embryo

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.772； 五年影响因子：4.933 ）

ISSN：

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Calla lily (Zantedeschia spp.) have great aesthetic value due to their spathe-like appearance and richness of coloration. However, embryonic callus regeneration is absent from its current regeneration mechanism. As a result, constructing an adequate and stable genetic transformation system is hampered, severely hindering breeding efforts. In this research, the callus induction effectiveness of calla lily seed embryos of various maturities was evaluated. The findings indicated that mature seed embryos were more suitable for in vitro regeneration. Using orthogonal design experiments, the primary elements influencing in vitro regeneration, such as plant growth regulators, genotypes, and nanoscale materials, which was emergent uses for in vitro regeneration, were investigated. The findings indicated that MS supplemented with 6-BA 2 mg/L and NAA 0.1 mg/L was the optimal medium for callus induction (CIM); the germination medium (GM) was MS supplemented with 6-BA 2 mg/L NAA 0.2 mg/L and 1 mg/L CNTs, and the rooting medium (RM) was MS supplemented with 6-BA 2 mg/L NAA 0.7 mg/L and 2 mg/L CNTs. This allowed us to verify, in principle, that the Agrobacterium tumefaciens-mediated genetic transformation system operates under optimal circumstances using the GUS reporter gene. Here, we developed a seed embryo-based genetic transformation regeneration system, which set the stage for future attempts to create new calla lily varieties.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台