Nano eggshell calcium enhanced gel properties of Nemipterus virgatus surimi sausage: gel strength, water retention and microstructure
文献类型： 外文期刊
作者： Huang, Qun 1 ; Huang, Xiang 1 ; Liu, Lan 2 ; Song, Hongbo 2 ; Geng, Fang 3 ; Wu, Wenjin 4 ; Luo, Peng 1 ;
作者机构： 1.Guizhou Med Univ, Sch Publ Hlth, Key Lab Environm Pollut Monitoring & Dis Control, Minist Educ, Guiyang 550025, Peoples R China
2.Fujian Agr & Forestry Univ, Coll Food Sci, Engn Res Ctr Fujian Taiwan Special Marine Food Pr, Minist Educ, Fuzhou 350002, Fujian, Peoples R China
3.Chengdu Univ, Sch Food & Biol Engn, Meat Proc Key Lab Sichuan Prov, Chengdu 610106, Sichuan, Peoples R China
4.Hubei Acad Agr Sci, Inst Agr Prod Proc & Nucl Agr Technol, Wuhan 430064, Peoples R China
5.Guizhou Med Univ, Guian New Dist 550025, Guizhou, Peoples R China
关键词： Gel micromorphology; gel texture; nano eggshell calcium; protein conformation; surimi; transglutaminase
期刊名称：INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY （ 影响因子：3.713； 五年影响因子：3.408 ）
ISSN： 0950-5423
年卷期： 2021 年 56 卷 11 期
页码：
收录情况： SCI
摘要： The effects of nano eggshell calcium (NEC) at different levels (0-1.5 g/100 g) on the gel properties of Nemipterus virgatus surimi were investigated. The results indicated that under the condition of adding 1.0 g/100 g, the gel strength of surimi was the highest, the water-holding capacity (WHC) was the best, and the cooking loss was the lowest. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that the addition of NEC promoted the covalent cross-linking between myosin heavy chains (MHC). Raman spectroscopy demonstrated that low contents (0.5-1.0 g/100 g) of NEC promoted the conformational transition of surimi protein from alpha-helix to beta-sheet. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) showed that the surimi gel with 1.0 g/100 g NEC had a denser network structure. Nevertheless, in the mixed gel with 1.25-1.5 g/100 g NEC, the network structure of the mixed-protein matrix was disrupted, and the mechanical and physicochemical parameters were deteriorated.
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