Development and Characterisation of an Immortal Cell Line From Largemouth Bass (Micropterus salmoides) for Viral Studies
文献类型： 外文期刊
作者： Mai, Qianyi 1 ; Wang, Qing 1 ; Li, Yingying 1 ; Yin, Jiyuan 1 ; Mo, Xubing 1 ; Shi, Cunbin 1 ; Ren, Yan 1 ; Liu, Donghai 1 ; Liu, Xuanming 1 ; Sun, Dongli 2 ; Liu, Weiqiang 2 ; Jin, Yuqi 2 ; Zeng, Weiwei 2 ; Wang, Yingying 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Guangdong Prov Key Lab Aquat Anim Immunol & Sustai, Guangzhou, Peoples R China
2.Foshan Univ, Sch Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise B, Foshan, Peoples R China
关键词： largemouth bass; MSF cell line; virology
期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.2； 五年影响因子：2.2 ）
ISSN： 0140-7775
年卷期： 2025 年 48 卷 4 期
页码：
收录情况： SCI
摘要： Largemouth bass (Micropterus salmoides), a freshwater fish species, is cultivated widely across China. The industry has been greatly affected by various viral diseases. We generated a new immortal cell line from the fin of M. salmoides (MSF). It has been successfully maintained in continuous culture for over 80 passages. Cells multiply well at 28 degrees C using Medium 199 or Leibovitz's L-15 supplemented with 10%-20% fetal bovine serum. MSF cells comprise a mass of epithelialoid cells. Chromosome analysis showed that ploidy at passage 55 was 2n = 58. Amplification and analysis of the sequence of cytochrome oxidase I gene ascertained the origin of MSF cells from M. salmoides. The cell line is devoid of contamination with mycoplasma. Successful transfection with a GFP reporter gene indicated that the MSF cell line can be a useful tool for further gene expression studies. MSF cells exhibited susceptibility to Largemouth bass ranavirus (LMBV), Infectious spleen and kidney necrosis virus (ISKNV), and Siniperca chuatsi rhabdovirus (SCRV). Transmission electron microscopy (TEM), and immunofluorescence confirmed that the MSF cell line showed high susceptibility to all the tested viruses. The titre values of MSF after infection with LMBV, ISKNV, and SCRV reached 107.4 TCID50/mL, 106.15 TCID50/mL, and 106.35 TCID50/mL at 7dpi, respectively. In conclusion, an immortal cell line derived from the fin tissue of M. salmoides was successfully developed and certain fish viruses can effectively propagated in the newly established MSF cell line that can be helpful for future gene manipulation and infectious viral mechanistic studies in the future.
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