文献类型： 外文期刊

作者： Chen, Lian-Ge ¹ ; Lan, Tianlong ² ; Zhang, Shuo ¹ ; Zhao, Mengkai ⁶ ; Luo, Guangyu ⁷ ; Gao, Yi ² ; Zhang, Yuliang ¹ ; Du, Qingwei ¹ ; Lu, Houze ¹⁰ ; Li, Bimeng ² ; Jiao, Bingke ¹ ; Hu, Zhangli ⁶ ; Ma, Yingxin ⁷ ; Zhao, Qiao ⁷ ; Wang, Ying ² ; Qian, Wenfeng ¹ ; Dai, Junbiao ⁶ ; Jiao, Yuling ¹ ;

作者机构： 1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing, Peoples R China

2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China

3.Peking Univ, Peking Univ Tsinghua Univ Natl Inst Biol Sci Joint, Acad Adv Interdisciplinary Studies, Beijing, Peoples R China

4.Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing, Peoples R China

5.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China

6.Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen, Peoples R China

7.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Guangdong Prov Key Lab Synthet Genom,CAS Key Lab Q, Shenzhen, Peoples R China

8.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Shenzhen Key Lab Synthet Genom, Shenzhen, Peoples R China

9.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing, Peoples R China

10.Peking Univ, Sch Earth & Space Sci, Beijing, Peoples R China

11.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Genome Anal Lab, Minist Agr & Rural Affairs,Guangdong Lab Lingnan M, Shenzhen, Peoples R China

12.Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Quantitat Biol, Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China

13.Peking Univ, Shandong Lab Adv Agr Sci Weifang, Inst Adv Agr Sci, Weifang, Peoples R China

期刊名称：NATURE PLANTS （ 影响因子：18.0； 五年影响因子：18.6 ）

ISSN： 2055-026X

年卷期： 2024 年 10 卷 2 期

页码：

收录情况： SCI

摘要： Rapid advances in DNA synthesis techniques have enabled the assembly and engineering of viral and microbial genomes, presenting new opportunities for synthetic genomics in multicellular eukaryotic organisms. These organisms, characterized by larger genomes, abundant transposons and extensive epigenetic regulation, pose unique challenges. Here we report the in vivo assembly of chromosomal fragments in the moss Physcomitrium patens, producing phenotypically virtually wild-type lines in which one-third of the coding region of a chromosomal arm is replaced by redesigned, chemically synthesized fragments. By eliminating 55.8% of a 155 kb endogenous chromosomal region, we substantially simplified the genome without discernible phenotypic effects, implying that many transposable elements may minimally impact growth. We also introduced other sequence modifications, such as PCRTag incorporation, gene locus swapping and stop codon substitution. Despite these substantial changes, the complex epigenetic landscape was normally established, albeit with some three-dimensional conformation alterations. The synthesis of a partial multicellular eukaryotic chromosome arm lays the foundation for the synthetic moss genome project (SynMoss) and paves the way for genome synthesis in multicellular organisms. Genome synthesis is moving into the multicellular era. This study redesigned and replaced a 155 kb chromosomal region with synthetic sequence in the model moss Physcomitrium patens. The simplified sequence obtains the correct epigenetic landscape.