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Gene mapping and candidate gene analysis of a sorghum sheathed panicle-I mutant

文献类型: 外文期刊

作者: Ao, Jianling 1 ; Wang, Ruoruo 3 ; Li, Wenzeng 2 ; Ding, Yanqing 2 ; Xu, Jianxia 2 ; Cao, Ning 2 ; Gao, Xu 2 ; Cheng, Bin 2 ; Zhao, Degang 3 ; Zhang, Liyi 2 ;

作者机构: 1.Guizhou Univ, Coll Life Sci, Guiyang, Peoples R China

2.Guizhou Acad Agr Sci, Guizhou Inst Upland Crops, Guiyang 550006, Peoples R China

3.Guizhou Acad Agr Sci, Biotechnol Inst Guizhou Prov, Plant Conservat Technol Ctr, Guizhou Key Lab Agr Biotechnol, Guiyang, Peoples R China

4.Guizhou Univ, Key Lab Plant Resource Conservat & Germplasm Innov, Minist Educ, Guiyang, Peoples R China

期刊名称:PLANT GENOME ( 影响因子:3.8; 五年影响因子:4.2 )

ISSN:

年卷期: 2025 年 18 卷 1 期

页码:

收录情况: SCI

摘要: Panicle exsertion is essential for crop yield and quality, and understanding its molecular mechanisms is crucial for optimizing plant architecture. In this study, the sheathed panicle-I (shp-I) mutant was identified from the ethyl methane sulfonate mutant population of the sorghum [Sorghum bicolor (L.) Moench] variety Hongyingzi (HYZ). While phenotypically similar to the wild type during the seedling stage, shp-I exhibits a significantly shorter peduncle internode at the heading stage. Cytomorphological analysis revealed reduced parenchyma cell size within the mutant's peduncle internode. Phytohormonal profiling showed lower levels of indole-3-acetic acid and higher concentrations of brassinosteroid in the mutant compared to the wild type at the peduncle internode. Genetic analysis confirmed that the mutant phenotype was caused by a recessive single-gene mutation. Through bulked segregant analysis sequencing (BSA-seq) genetic mapping, the causative locus for the mutant phenotype was localized to a 59.65-59.92 Mb interval on chromosome 10, which contains 28 putative genes. Additionally, the gene SbiHYZ.10G230700, which encodes a BTB/POZ and MATH (BPM) domain protein, was identified as a candidate gene. Further analysis revealed that the non-synonymous mutations in the candidate gene were located within the MATH domain, affecting the 3D structure of the protein. In summary, this study provides a new genetic material and candidate genes for future research into the molecular regulation of sorghum peduncle length.

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