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Molecular cloning of a specific expression gene for cold acclimation in cucumber

文献类型： 外文期刊

作者： Kang, GB 1 ; Xu, Y 2 ; Yong, WD 2 ; Ge, L 2 ; Wang, LP 2 ; Zhang, HY 2 ; Wang, YJ 2 ; Chong, K 2 ;

作者机构： 1.Natl Engn Res Ctr Vegetables, Beijing 100089, Peoples R China

2.Natl Engn Res Ctr Vegetables, Beijing 100089, Peoples R China; Chinese Acad Sci, Inst Bot, Res Ctr Mol & Dev Biol, Beijing 100093, Peoples R China

关键词： Cucumis sativus; cold acclimation; cDNA clone; mRNA differential display

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN： 0577-7496

年卷期： 2001 年 43 卷 9 期

页码：

收录情况： SCI

摘要： A strategy of mRNA differential display was used for screening specific expression genes for cold acclimation in cucumber ( Cucumis sativus L.). "Jinyan No. 4", a cold sensitive cultivar Of cucumber, was treated under the conditions of 8 degreesC for 0, 6, 12, 24, 48 and 72 h, respectively, once it grew until two true leaves stage. A cold specific cDNA clone named as ccr18 was obtained. Data of differential display and Northern blot analysis showed that it expressed at cold acclimation for 12 li or more, and the longer duration at cold condition the higher it expressed. Southern blot assay suggested that it was a gene with single copy in chromosome of cucumber. Sequencing data showed the size of the cDNA clone was 639 bp in length. It shared 88% sequence with a BAC F14P3 genomic sequence of Arabidopsis thaliana chromosome III at protein sequence level.

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