文献类型： 外文期刊
作者： Tian, Luming 1 ; Huang, Conglin 2 ; Yu, Rong 2 ; Liang, Ruifang 2 ; Li, Zhiliang 2 ; Zhang, Lusheng 2 ; Wang, Yongqin; 1 ;
作者机构： 1.Beijing Res Ctr Agrobiotechnol, Beijing 100089, Peoples R China
2.Beijing Res Ctr Agrobiotechnol, Beijing 100089, Peoples R China; Capital Normal Univ, Coll Life Sci, Beijing 100037, Peoples R China; China Agr Univ, Coll Agron & Biotechnol, Beijing 100094, Peoples R China
关键词： AtNHX1 gene; transgenic tall fescue; particle bombardment; salt-tolerance
期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）
ISSN： 1684-5315
年卷期： 2006 年 5 卷 11 期
页码：
收录情况： SCI
摘要： Saline soil is a serious problem worldwide, and it is necessary to improve the salt tolerance of plants so as to avoid the progressive deterioration of saline soil. Here we report that over-expression of AtNHX1 improves salt tolerance in transgenic tall fescue. The AtNHX1 gene driven with CaMV35S promoter was constructed into the plant expression vector pGreen0229, and introduced into the embryonic calli of hypocotyls of tall fescue (Festuca arundinacea) by particle bombardment. Regenerated plantlets were obtained by screening of herbicide (PPT, 2 mg/L), and the putative transformants were assayed by PCR and western blot analysis. 29 transgenic plants were obtained. The results indicated that the exogenous genes had been integrated into the genomes of transgenic plants, and AtNHX1 is expressed in the plants. There was remarkable salt tolerance in transgenic plants compared to control plants.
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