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Overexpression of copper/zinc superoxide dismutase from mangrove Kandelia candel in tobacco enhances salinity tolerance by the reduction of reactive oxygen species in chloroplast

文献类型： 外文期刊

作者： Jing, Xiaoshu 1 ; Hou, Peichen 2 ; Lu, Yanjun 1 ; Deng, Shurong 1 ; Li, Niya 3 ; Zhao, Rui 1 ; Sun, Jian 4 ; Wang, Yang 1 ;

作者机构： 1.Beijing Forestry Univ, Coll Biol Sci & Technol, Dept Plant Sci, Beijing 100083, Peoples R China

2.Natl Engn Res Ctr Informat Technol Agr, Dept Bio Instruments, Beijing, Peoples R China

3.Hainan Normal Univ, Coll Life Sci, Dept Biol, Haikou, Peoples R China

4.Jiangsu Normal Univ, Coll Life Sci, Dept Plant Sci, Xuzhou, Peoples R China

5.Zhejiang Agr & Forestry Univ, Coll Agr & Food Sci, Dept Crop Sci, Hangzhou, Zhejiang, Peoples R China

关键词： Kandelia candel; Na+ flux; superoxide anion; hydrogen peroxide; salt; catalase; superoxide dismutase

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2015 年 6 卷

页码：

收录情况： SCI

摘要： Na+ uptake and transport in Kandelia candel and antioxidative defense were investigated under rising NaCI stress from 100 to 300 mM. Salinized K. candel roots had a net Na+ efflux with a declined flux rate during an extended NaCI exposure. Na+ buildup in leaves enhanced H2O2 levels, superoxide dismutase (SOD) activity, and increased transcription of CSD gene encoding a Cu/Zn SOD. Sequence and subcellular localization analyses have revealed that KcCSD is a typical Cu/Zn SOD in chloroplast. The transgenic tobacco experimental system was used as a functional genetics model to test the effect of KcCSD on salinity tolerance. KcCSD-transgenic lines were more Na+ tolerant than wildtype (WT) tobacco in terms of lipid peroxidation, root growth, and survival rate. In the latter, 100 mM NaCI led to a remarkable reduction in chlorophyll content and a/b ratio, decreased maximal chlorophyll a fluorescence, and photochemical efficiency of photosystem II. NaCI stress in WT resulted from H2O2 burst in chloroplast. Na+ injury to chloroplast was less pronounced in KcCSD-transgenic plants due to upregulated antioxidant defense. KcCSD-transgenic tobacco enhanced SOD activity by an increment in SOD isoenzymes under 100 mM NaCI stress from 24h to 7 day. Catalase activity rose in KcCSD overexpressing tobacco plants. KcCSD-transgenic plants better scavenged NaCl-elicited reactive oxygen species (ROS) compared to WT ones. In conclusion, K. candel effectively excluded Na+ in roots during a short exposure; and increased CSD expression to reduce ROS in chloroplast in a long-term and high saline environment.

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