Development of a core set of KASP markers for assaying genetic diversity in Brassica rapa subsp. chinensis Makino
文献类型： 外文期刊
作者： Li, Peirong 1 ; Su, Tongbing 1 ; Wang, Huiping 1 ; Zhao, Xiuyun 1 ; Wang, Weihong 1 ; Yu, Yangjun 1 ; Zhang, Deshuang; 1 ;
作者机构： 1.BAAFS, BVRC, Beijing, Peoples R China
2.Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing, Peoples R China
3.Beijing Key Lab Vegetable Germplasm Improvement, Beijing, Peoples R China
关键词： genetic diversity; KASP assays; molecular markers; non-heading Chinese cabbage; single-nucleotide polymorphism (SNP)
期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）
ISSN： 0179-9541
年卷期： 2019 年 138 卷 3 期
页码：
收录情况： SCI
摘要： Single-nucleotide polymorphisms (SNPs) are rapid, economical and reliable genotyping tools. Non-heading Chinese cabbage (Brassica rapa L. subsp. chinensis Makino) is now an economically important vegetable crop worldwide. In this study, 1,167 SNPs were evaluated for 7polymorphism among 70 representative non-heading Chinese cabbage inbred lines using a Kompetitive Allele Specific PCR (KASP) genotyping assay. On the basis of identified polymorphisms and the results of a principal component analysis, we selected 50 core SNPs that were balanced sufficiently to provide adequate information for genetic identification. The core SNPs were used for construction of a neighbour-joining dendrogram that separated the 70 inbred lines into four main groups and several subgroups corresponding to Caixin, Heiyebaicai, Huangxinwu, Naibaicai, Taitsai, Pak-choi, and Wutatsai. This categorization was superior to that achieved using a dataset of 479 polymorphic SNPs. To confirm the utility of the core SNP markers in genetic identification, we tested their stability and resolution using 162 commercial hybrid cultivars. The SNPs, which represent a cost-effective, accurate marker set for germplasm analysis and cultivar identification, are suitable for molecular marker-assisted breeding in non-heading Chinese cabbage.
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