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Elucidating Escherichia Coli O157:H7 Colonization and Internalization in Cucumbers Using an Inverted Fluorescence Microscope and Hyperspectral Microscopy

文献类型： 外文期刊

作者： Sun, Yeting 1 ; Wang, Dan 2 ; Ma, Yue 2 ; Guan, Hongyang 1 ; Liang, Hao 3 ; Zhao, Xiaoyan 1 ;

作者机构： 1.Shenyang Agr Univ, Coll Food Sci, Shenyang 110866, Liaoning, Peoples R China

2.Minist Agr & Rural Affairs, Beijing Vegetable Res Ctr, Beijing Key Lab Agr Prod Fruits & Vegetables Pres, Key Lab Vegetable Postharvest Proc,Beijing Acad A, Beijing 100097, Peoples R China

3.Longda Food Grp Co Ltd, Jinan 265231, Shandong, Peoples R China

关键词： cucumbers; Escherichia coli O157:H7; hyperspectral microscopy; colonization; internalization

期刊名称：MICROORGANISMS （ 影响因子：4.128； ）

ISSN：

年卷期： 2019 年 7 卷 11 期

页码：

收录情况： SCI

摘要： Contamination of fresh cucumbers (Cucumis sativus L.) with Escherichia coli O157:H7 can impact the health of consumers. Despite this, the pertinent mechanisms underlying E. coli O157:H7 colonization and internalization remain poorly documented. Herein we aimed to elucidate these mechanisms in cucumbers using an inverted fluorescence microscope and hyperspectral microscopy. We observed that E. coli O157:H7 primarily colonized around the stomata on cucumber epidermis without invading the internal tissues of intact cucumbers. Once the bacterial cells had infiltrated into the internal tissues, they colonized the cucumber placenta and vascular bundles (xylem vessels, in particular), and also migrated along the xylem vessels. Moreover, the movement rate of E. coli O157:H7 from the stalk to the flower bud was faster than that from the flower bud to the stalk. We then used hyperspectral microscope imaging to categorize the infiltrated and uninfiltrated areas with high accuracy using the spectral angle mapper (SAM) classification method, which confirmed the results obtained upon using the inverted fluorescence microscope. We believe that our results are pivotal for developing science-based food safety practices, interventions for controlling E. coli O157:H7 internalization, and new methods for detecting E. coli O157:H7-plant interactions.

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