您好，欢迎访问湖北省农业科学院 机构知识库！
湖北省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

MfOfd1 is crucial for stress responses and virulence in the peach brown rot fungus Monilinia fructicola

文献类型： 外文期刊

作者： Zhang, Ming-Ming 1 ; Wang, Zuo-Qian 2 ; Xu, Xiao 1 ; Huang, Song 1 ; Yin, Wei-Xiao 3 ; Luo, Chao-Xi 1 ;

作者机构： 1.Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ, Wuhan 430070, Peoples R China

2.Hubei Acad Agr Sci, Inst Plant Protect & Soil Fertilizer, Wuhan, Peoples R China

3.Huazhong Agr Univ, Key Lab Crop Dis Monitoring & Safety Control Hube, Wuhan, Peoples R China

4.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Peoples R China

关键词： exogenous stresses; MfOfd1 gene; Monilinia fructicola; redox response; sporulation; virulence

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：5.663； 五年影响因子：5.626 ）

ISSN： 1464-6722

年卷期： 2020 年 21 卷 6 期

页码：

收录情况： SCI

摘要： Monilinia fructicola is the most widely distributed species among the Monilinia genus in the world, and causes blossom blight, twig canker, and fruit rot on Rosaceae fruits. To date, studies on genomics and pathogenicity are limited in M. fructicola. In this study, we identified a redox-related gene, MfOfd1, which was significantly up-regulated at 1 hr after inoculation of M. fructicola on peach fruits. We used the clustered regulatory inter-spaced short palindromic repeats (CRISPR)/Cas9 system combined with homologous recombination to determine the function of the MfOfd1 gene. The results showed that the sporulation of knockdown transformants was reduced by 53% to 83%. The knockdown transformants showed increased sensitivity to H2O2 and decreased virulence on peach fruits compared to the wild-type isolate Bmpc7. It was found that H2O2 could stimulate the expression of MfOfd1 in the wild-type isolate. The transformants were also more sensitive to exogenous osmotic stress, such as glycerol, d-sorbitol, and NaCl, and to dicarboximide fungicides (iprodione and dimethachlon). These results indicate that the MfOfd1 gene plays an important role in M. fructicola in sporulation, oxidative response, osmotic stress tolerance, and virulence.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖北省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台