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Functional analysis of a miRNA-like small RNA derived from Bombyx mori cytoplasmic polyhedrosis virus

文献类型： 外文期刊

作者： Guo, Jian-Yong 1 ; Wang, Yong-Sheng 1 ; Chen, Tian 1 ; Jiang, Xiao-Xu 1 ; Wu, Ping 1 ; Geng, Tao 3 ; Pan, Zhong-Hua 4 ; S 1 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Zhenjiang 212018, Jiangsu, Peoples R China

2.Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Jiangsu, Peoples R China

3.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikoou, Peoples R China

4.Soochow Univ, Sch Biol & Basic Med Sci, Suzhou, Peoples R China

关键词： Bombyx mori; cytoplasmic polyhedrosis virus; inhibitor of apoptosis protein; miRNA-like small RNA

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期： 2020 年 27 卷 3 期

页码：

收录情况： SCI

摘要： Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is a major pathogen of the economic insect silkworm, Bombyx mori. Virus-encoded microRNAs (miRNAs) have been proven to play important roles in host-pathogen interactions. In this study we identified a BmCPV-derived miRNA-like 21 nt small RNA, BmCPV-miR-1, from the small RNA deep sequencing of BmCPV-infected silkworm larvae by stem-loop quantitative real-time PCR (qPCR) and investigated its functions with qPCR and lentiviral expression systems. Bombyx mori inhibitor of apoptosis protein (BmIAP) gene was predicted by both target prediction software miRanda and Targetscan to be one of its target genes with a binding site for BmCPV-miR-1 at the 5 ' untranslated region. It was found that the expression of BmCPV-miR-1 and its target gene BmIAP were both up-regulated in BmCPV-infected larvae. At the same time, it was confirmed that BmCPV-miR-1 could up-regulate the expression of BmIAP gene in HEK293T cells with lentiviral expression systems and in BmN cells by transfecting mimics. Furthermore, BmCPV-miR-1 mimics could up-regulate the expression level of BmIAP gene in midgut and fat body in the silkworm. In the midgut of BmCPV-infected larvae, BmCPV-miR-1 mimics could be further up-regulated and inhibitors could lower the virus-mediated expression of BmIAP gene. With the viral genomic RNA segments S1 and S10 as indicators, BmCPV-miR-1 mimics could up-regulate and inhibitors down-regulate their replication in the infected silkworm. These results implied that BmCPV-miR-1 could inhibit cell apoptosis in the infected silkworm through up-regulating BmIAP expression, providing the virus with a better cell circumstance for its replication.

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