文献类型： 外文期刊

作者： Ashrae, Muhammad Nadeem ¹ ; Hu, Cheng ² ; Wu, Lei ¹ ; Duan, Yinghua ¹ ; Zhang, Wenju ¹ ; Aziz, Tariq ³ ; Cai, Andong ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Natl Engn Lab Improving Qual Arable Land, Beijing 100081, Peoples R China

2.Hubei Acad Agr Sci, Inst Plant Protect & Soil Sci, Wuhan 430064, Peoples R China

3.Univ Agr Faisalabad, Inst Soil & Environm Sci, Faisalabad 38040, Pakistan

4.Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Key Lab Agroenvironm, Minist Agr & Rural Affairs, Beijing 100081, Peoples R China

关键词： Stoichiometry; SOC mineralization; Nitrogen mineralization; Microbial biomass; Nutrient availability; Long-term fertilization

期刊名称：JOURNAL OF SOILS AND SEDIMENTS （ 影响因子：3.308； 五年影响因子：3.586 ）

ISSN： 1439-0108

年卷期： 2020 年 20 卷 8 期

页码：

收录情况： SCI

摘要： Purpose Soil microbial biomass (SMB), as the source and sink of soil nutrients, and its stoichiometry play a key role in soil organic carbon (SOC) and nitrogen (N) mineralization. The objective of this study was to investigate the responses of SOC and N mineralization to changes in microbial biomass and SOC, N, and phosphorus (P) stoichiometry resulted from long-term fertilization regimes. Materials and methods Soil was sampled from a rice-wheat rotation system subjected to 37 years of nine fertilization treatments with different nutrient input amounts: control (CK), N alone, N combined with mineral phosphorus (NP), NP plus potassium (NPK), manure alone (M), and M combined with N (MN), NP (MNP), NPK (MNPK), and a higher rate of M with NPK (hMNPK). The sampled soil was incubated for the determination of SOC and N mineralization, C, N, and P stoichiometry of soil and SMB, and associated soil enzymes related to C and N cycling. Results and discussion Relative to the CK and treatments with mineral fertilizers, treatments with manure (M, MN, MNP, MNPK, and hMNPK) significantly increased SOC and N mineralization by 48-78% and 54-97%, respectively. Microbial metabolic quotient (qCO(2)) decreased by 32-55% in treatments with manure compared to the N and NP treatments, but showed no effect on the qCO(2) when compared to the NPK treatment. The leucine amino peptidase (LAP) enzyme showed significant positive correlation with SOC and N mineralization, and negatively related to the qCO(2). Significantly negative correlations were also observed between SOC and N mineralization and soil C:P and N:P ratio, as well as microbial biomass SMBC:SMBP and SMBN:SMBP stoichiometry, respectively. However, the availability of N and P had limited effects on the qCO(2) after reaching a certain value (0.69-0.72 mg CO2-C g(-1) MBC h(-1)). Conclusions Lower soil elemental (C:P and N:P) and microbial biomass stoichiometry (SMBC:SMBP and SMBN:SMBP) and increase of LAP resulted from combined application of manure and mineral fertilizers, accelerated SOC, and N mineralization. Mineral nutrient input with manure amendments could be an optimal strategy to meet the microbial stoichiometric demands and enhance nutrient availability for crops in agricultural ecosystems.