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Determination of Aflatoxin B-1 in Lotus Seed by High Performance Liquid Chromatography with Aptamer Affinity Column for Purification and Enrichment

文献类型： 外文期刊

作者： Zhao Ying 1 ; Wang Nan 1 ; Gao Hua-Long 2 ; Guo Zi-Xuan 2 ; Lu An-Xiang 2 ; Guo Xiao-Jun 2 ; Lu Jing-Hua 1 ; Luan Yun-Xia 2 ;

作者机构： 1.Jinzhou Med Univ, Coll Pharm, Jinzhou 121001, Peoples R China

2.Beijing Res Ctr Agr Stand & Testing, Agr Prod Qual & Safety Risk Assessment Lab, Beijing Municipal Key Lab Agr Environm Monitoring, Dept Agr Beijing, Beijing 100097, Peoples R China

3.Capital Normal Univ, Beijing 100097, Peoples R China

关键词： Aptamer affinity column; Aflatoxin B-1; Solid-phase extraction; N-Hydroxy succinimide-activated sepharose; Lotus seed

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2020 年 48 卷 5 期

页码：

收录情况： SCI

摘要： An aptamer affinity column (AAC) was applied to specific identification and enrichment of aflatoxin B-1 (AFB(1)). Aptamer was used as a novel recognition element for the high affinity and strong specificity of target. By optimizing the loading, washing and eluting conditions of AFB, in the sample, AFB(1) aptamer affinity column exhibited a good sample capacity (334.6 +/- 18.2) ng. The content of AFB(1) lotus seeds was detected by photo chemical reactor for enhanced high-performance liquid chromatography combined with photo chemical reactor for enhanced detection and fluorescence detection (HPLC-PCD-FLD) and AAC cleanup, with LOD of 0.05 ng/mL and excellent recoveries of 91.8% -108. 6% . Meanwhile, the AAC could be reused at least twenty times. This study provides a new method 14 the enrichment and purification of trace toxins in complex matrix such as food, agricultural products and traditional Chinese medicines , and has a good application prospect.

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