Cloning, Secretory Expression and Characterization of a Unique pH-Stable and Cold-Adapted Alginate Lyase
文献类型： 外文期刊
作者： Wang, Zhi-Peng 1 ; Cao, Min 2 ; Li, Bing 2 ; Ji, Xiao-Feng 1 ; Zhang, Xin-Yue 2 ; Zhang, Yue-Qi 2 ; Wang, Hai-Ying 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, Qingdao 266071, Peoples R China
2.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao 266109, Peoples R China
3.Yellow Sea Fisheries Res Inst, Lab Enzyme Engn, Qingdao 266071, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266071, Peoples R China
关键词： alginate lyase; cold-adapted; pH-stable; NaCl-independent
期刊名称：MARINE DRUGS （ 影响因子：5.118； 五年影响因子：5.951 ）
ISSN：
年卷期： 2020 年 18 卷 4 期
页码：
收录情况： SCI
摘要： Cold-adapted alginate lyases have unique advantages for alginate oligosaccharide (AOS) preparation and brown seaweed processing. Robust and cold-adapted alginate lyases are urgently needed for industrial applications. In this study, a cold-adapted alginate lyase-producing strain Vibrio sp. W2 was screened. Then, the gene ALYW201 was cloned from Vibrio sp. W2 and expressed in a food-grade host, Yarrowia lipolytica. The secreted Alyw201 showed the activity of 64.2 U/mL, with a molecular weight of approximate 38.0 kDa, and a specific activity of 876.4 U/mg. Alyw201 performed the highest activity at 30 degrees C, and more than 80% activity at 25-40 degrees C. Furthermore, more than 70% of the activity was obtained in a broad pH range of 5.0-10.0. Alyw201 was also NaCl-independent and salt-tolerant. The degraded product was that of the oligosaccharides of DP (Degree of polymerization) 2-6. Due to its robustness and its unique pH-stable property, Alyw201 can be an efficient tool for industrial production.
- 相关文献
作者其他论文 更多>>
-
Comparison of Intestinal Bacteria of Procambarus clarkii Farmed in Various Rice Paddy Regions
作者：Ding, Chonghang;Zhu, Jian;Jia, Rui;Hou, Yiran;Zhang, Liqiang;Li, Bing;Zhu, Jian;Wang, Yunfeng
关键词：Procambarus clarkii; integrated rice-crayfish co-culture; intestinal microbiota; microbial diversity; co-occurrence networks
-
Variations in antibiotic resistomes associated with archaeal, bacterial, and viral communities affected by integrated rice-fish farming in the paddy field ecosystem
作者：Hou, Yiran;Jia, Rui;Sun, Wei;Feng, Wenrong;Li, Bing;Zhu, Jian;Hou, Yiran;Diao, Weixu;Jia, Rui;Feng, Wenrong;Li, Bing;Zhu, Jian
关键词：Antibiotic resistomes; Antibiotic resistance genes (ARGs); Agricultural production patterns; Clean and sustainable agricultural production; Integrated rice-fish farming; Paddy field; Archaeal; Bacterial; And viral communities
-
Influences of the Integrated Rice-Crayfish Farming System with Different Stocking Densities on the Paddy Soil Microbiomes
作者：Hou, Yiran;Jia, Rui;Sun, Wei;Li, Bing;Zhu, Jian;Hou, Yiran;Jia, Rui;Li, Bing;Zhu, Jian
关键词：red claw crayfish; stocking density; paddy field; soil bacterial community; agricultural production patterns
-
Effects of Berberine on Lipid Metabolism, Antioxidant Status, and Immune Response in Liver of Tilapia (Oreochromis niloticus) under a High-Fat Diet Feeding
作者：Jia, Rui;Hou, Yiran;Zhang, Liqiang;Li, Bing;Zhu, Jian;Jia, Rui;Hou, Yiran;Zhang, Liqiang;Li, Bing;Zhu, Jian
关键词：berberine; high-fat diet; lipid metabolism; liver damage; Oreochromis niloticus
-
Effects of Different Stocking Densities on the Growth, Antioxidant Status, and Intestinal Bacterial Communities of Carp in the Rice-Fish Co-Culture System
作者：Diao, Weixu;Jia, Rui;Hou, Yiran;Gong, Jianyou;Zhang, Liqiang;Li, Bing;Zhu, Jian;Jia, Rui;Hou, Yiran;Zhang, Liqiang;Li, Bing;Zhu, Jian;Jia, Rui
关键词：common carp; integrated rice-carp co-culture system; oxidative stress; intestinal microbiota
-
Comprehensive analysis of circRNA-miRNA-mRNA networks in the kidney of snakehead (Channa argus) response to Nocardia seriolae challenge
作者：Zhang, Xiaoyan;Yu, Haohui;Wang, Ningning;Liu, Xiantong;Cao, Min;Li, Chao;Jiang, Yanliang
关键词：Snakehead(Channaargus); Nocardia seriolae; Kidney; circRNA-miRNA-mRNA networks; Immune response
-
Growth arrest and DNA damage-inducible 45 ab gene targeted by miR-20b-5p executes the inflammation and apoptosis by regulating MAPK and NF-κB pathways in black rockfish
作者：Gao, Chengbin;Cai, Xin;Zhu, Ying;Cao, Min;Li, Chao;Gao, Chengbin;Cai, Xin;Ma, Le;Sun, Peng
关键词：Ss _ gadd45ab; Inflammation; Apoptosis; Black rockfish; Regulatory mechanism
