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Molecular Functional Characterisation of MechlPPDK Promoter in Transgenic Tobacco

文献类型： 外文期刊

作者： Wang, Haiyan 1 ; Shen, Xu 3 ; Lu, Cheng 1 ; Li, Kaimian 1 ; Wang, Wenquan 1 ;

作者机构： 1.Chinese Acad Trop Agicultural Sci CATAS, Inst Trop Biosci & Biotechnol ITBB, Xueyuan Rd 4, Haikou 571101, Hainan, Peoples R China

2.Minist Agr, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Hainan, Peoples R China

3.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China

关键词： Abiotic stress; Manihot esculenta; Pyruvate phosphate dikinase; Promoter; Regulatory elements

期刊名称：TROPICAL PLANT BIOLOGY （ 影响因子：1.512； 五年影响因子：2.011 ）

ISSN： 1935-9756

年卷期： 2020 年 13 卷 3 期

页码：

收录情况： SCI

摘要： Chloroplastic pyruvate phosphate dikinase (PPDK) (chlPPDK) is a key enzyme in the photosynthesis of C4 plants. PPDK is expressed in high abundance in C4 plants but only in trace amounts in C3 plants. The existing research reveals a higher expression of MechlPPDK in cultivated cassava varieties than that in wild cassava W14. However, knowledge about the transcriptional regulation of the MechlPPDK gene in cassava (Manihot esculenta Crantz) is insufficient. Therefore, we aim to identify the transcription profile of MechlPPDK and the core promoter region of MechlPPDK. We cloned the MechlPPDK coding sequence and its 5 '-flanking sequence from the cassava variety Ku50. A series of deletion constructions fused to a uidA reporter gene were performed in the 5 '-flanking sequence and stably transformed into tobacco. We found that P1 (from -590 bp to +114 bp) had the highest promoter activity among P1 to P3. The 5 '-flanking sequence of MechlPPDK responded differently to varied abiotic stresses. Our results will further the understanding of the regulation of MechlPPDK expression.

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