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Rice virus release from the planthopper salivary gland is independent of plant tissue recognition by the stylet

文献类型： 外文期刊

作者： Xu, Chunling 1 ; Lu, Chengye 1 ; Piao, Jun 2 ; Wang, Yixiao 2 ; Zhou, Tong 1 ; Zhou, Yijun 1 ; Li, Shuo 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Minist Sci & Technol, State Key Lab Cultivat Base, Inst Plant Protect,Jiangsu Key Lab Food Qual & Sa, Nanjing, Peoples R China

2.Liaoning Normal Univ, Sch Life Sci, Dalian, Peoples R China

3.Ningbo Univ, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo, Peoples R China

4.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang, Jiangsu, Peoples R China

关键词： small brown planthopper; viral release mode; stylet sensing; EPG analysis; watery saliva proteins

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2020 年 76 卷 9 期

页码：

收录情况： SCI

摘要： Background The transmission of plant viruses by arthropod vectors is closely related to feeding behavior. For persistently transmitted viruses, virus release means that virus moves through the salivary gland microvillus barriers of insects into plant via the stylet. However, whether virus release is dependent on plant tissue and component recognition by the stylet is unclear. Results In this study, the small brown planthopper (SBPH) and two rice viruses transmitted by it were used as a model to explore this question. After the viruliferous insects penetrated a stretched membrane without plant tissue structure and ingested liquid food (rice sap, nutrient solution or water), both viruses were detected in the liquid food after only a 6 min inoculation access period, suggesting that the viruses were released from SBPH salivary gland independent of plant tissue and component recognition by the stylet. In subsequent electrical penetration graph (EPG) analysis, N4a-like and N4b-like waveforms, similar to N4a (phloem salivation before ingestion) and N4b (sieve element ingestion), were observed during SBPH penetrating the membrane, exhibiting normal feeding activity of planthopper on membrane, which further demonstrated that virus release from salivary gland was along with feeding activity, without the stylet sensing plant tissue. EPG analysis and identification of salivary proteins indicated more active feeding behavior and efficient salivation in viruliferous planthoppers. Conclusion These results suggest that the rice virus is released from insect salivary gland independent of plant tissue and component recognition by the stylet, and the simple virus release mode facilitates virus transmission by vectors.

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